Bockendahl H, Remy W, Remy B, Petersen G, Stüttgen G
Acta Derm Venereol. 1977;57(1):15-21.
In continuing our studies on the characteristics of the antigenic structures of the basement membrane, procedures used in histochemistry for the identification and blocking of reactive groups have been adapted in this present work to the pretreatment of cryostat sections of guinea-pig tongue. These sections have then been studied by the indirect immunofluorescence method. By means or reagents, which produce a reversible extinction of the basement membrane fluorescence produced with pemphigoid sera, it proved possible to determine the existence of certain chemical groupings which bind the anti-basement membrane antibodies, namely: free carboxyl groups deriving from amino acids, hydrogen bridges in which the hydroxyl groups from the carbohydrates in the antigen are probably involved, bonds involving the free aldehydes of the antigen (either cross-links from aldehydes, or formation of Schiff's bases with amino groups of the antibodies), and lastly, to a lesser extent, disulphide bonds. The antigen/antibody reaction probably occurs in the neighbourhood of the alpha-glucosido-beta-galactosido-hydroxylysine. A reaction model has been developed from these findings. There were also some indications that the antigenic structures of the intercellular substance are chemically related to those of the basement membrane. The cell nucleus antigens, on the other hand, appear to possess different chemical properties.
在继续我们关于基底膜抗原结构特征的研究中，本研究采用了组织化学中用于鉴定和封闭反应基团的方法，对豚鼠舌冷冻切片进行预处理。然后通过间接免疫荧光法对这些切片进行研究。通过使用能使类天疱疮血清产生的基底膜荧光发生可逆消退的试剂，证明有可能确定某些与抗基底膜抗体结合的化学基团的存在，即：来自氨基酸的游离羧基、可能涉及抗原中碳水化合物羟基的氢键、涉及抗原游离醛的键（醛的交联或与抗体氨基形成席夫碱），最后，二硫键的作用较小。抗原/抗体反应可能发生在α-葡糖苷-β-半乳糖苷-羟赖氨酸附近。根据这些发现建立了一个反应模型。也有一些迹象表明细胞间质的抗原结构与基底膜的抗原结构在化学上相关。另一方面，细胞核抗原似乎具有不同的化学性质。
