Taniuchi S, Tamoto K, Koyama J
J Biochem. 1978 Oct;84(4):907-15. doi: 10.1093/oxfordjournals.jbchem.a132203.
The usual anti-Ig antibody method, consisting of the precipitation of soluble antigen-antibody complexes by heterologous anti-Ig antibody, was applied for quantitative estimation of guinea pig IgG2 anti-ovalbumin and anti-2,4-dinitrophenyl (DNP) antibodies by measuring the maximum amounts of antibody-bound antigens. However, the amounts of antibodies estimated were less than those obtained by other methods: the precipitin reaction, the precipitation of antigen-antibody complexes with 50% saturated ammonium sulfate, and equilibrium dialysis. In particular, the anti-Ig antibody method greatly underestimated the amount of anti-DNP antibody with low affinity for epsilon-DNP-L-lysine. Thus, it was concluded that partial dissociation of the antigen-antibody complexes occurring upon precipitation with anti-Ig antibody made the anti-Ig antibody method unsuitable for quantitative determination of antibodies.
通常的抗Ig抗体方法，即通过异源抗Ig抗体沉淀可溶性抗原-抗体复合物，被用于通过测量抗体结合抗原的最大量来定量估算豚鼠IgG2抗卵清蛋白和抗2,4-二硝基苯基（DNP）抗体。然而，估算的抗体量比通过其他方法获得的量要少：沉淀素反应、用50%饱和硫酸铵沉淀抗原-抗体复合物以及平衡透析。特别是，抗Ig抗体方法极大地低估了对ε-DNP-L-赖氨酸亲和力低的抗DNP抗体的量。因此，得出的结论是，用抗Ig抗体沉淀时发生的抗原-抗体复合物的部分解离使得抗Ig抗体方法不适用于抗体的定量测定。
