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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Siskind G W, Paul W E, Benacerraf B

J Exp Med. 1966 Apr 1;123(4):673-88. doi: 10.1084/jem.123.4.673.

Fluorescence quenching has been calibrated, by comparison with equilibrium dialysis, for measurement of antibody-hapten association constants with guinea pig antibody to the 2,4-dinitrophenyl (DNP) determinant. The maximum quenching with all sites occupied which gave best agreement with equilibrium dialysis was found to be 100%. 2. The carrier to which the DNP-hapten is coupled influences significantly the amount, class (gamma(1) or gamma(2)), and affinity of the antibody of DNP specificity made by individual guinea pigs. 3. The affinities of gamma(1)- and gamma(2)-antibodies from individual guinea pigs are generally very similar. 4. Precipitin curves with gamma(1)-antibodies show more marked inhibition of precipitation in antigen excess than do precipitin curves with the less charged gamma(2)-antibodies, indicating the importance of nonspecific forces in the precipitation reactions.

通过与平衡透析法对比，已对荧光猝灭进行校准，用于测量豚鼠抗2,4 -二硝基苯基（DNP）决定簇抗体与半抗原的结合常数。发现所有位点被占据时的最大猝灭率为100%，这与平衡透析法最为吻合。2. 与DNP -半抗原偶联的载体显著影响个体豚鼠产生的DNP特异性抗体的量、类别（γ(1)或γ(2)）和亲和力。3. 个体豚鼠的γ(1) -抗体和γ(2) -抗体的亲和力通常非常相似。4. 与电荷较少的γ(2) -抗体的沉淀曲线相比，γ(1) -抗体的沉淀曲线在抗原过量时显示出对沉淀的更显著抑制，表明非特异性作用力在沉淀反应中的重要性。

Siskind G W, Paul W E, Benacerraf B

J Exp Med. 1966 Apr 1;123(4):673-88. doi: 10.1084/jem.123.4.673.

Fluorescence quenching has been calibrated, by comparison with equilibrium dialysis, for measurement of antibody-hapten association constants with guinea pig antibody to the 2,4-dinitrophenyl (DNP) determinant. The maximum quenching with all sites occupied which gave best agreement with equilibrium dialysis was found to be 100%. 2. The carrier to which the DNP-hapten is coupled influences significantly the amount, class (gamma(1) or gamma(2)), and affinity of the antibody of DNP specificity made by individual guinea pigs. 3. The affinities of gamma(1)- and gamma(2)-antibodies from individual guinea pigs are generally very similar. 4. Precipitin curves with gamma(1)-antibodies show more marked inhibition of precipitation in antigen excess than do precipitin curves with the less charged gamma(2)-antibodies, indicating the importance of nonspecific forces in the precipitation reactions.

通过与平衡透析法对比，已对荧光猝灭进行校准，用于测量豚鼠抗2,4 -二硝基苯基（DNP）决定簇抗体与半抗原的结合常数。发现所有位点被占据时的最大猝灭率为100%，这与平衡透析法最为吻合。2. 与DNP -半抗原偶联的载体显著影响个体豚鼠产生的DNP特异性抗体的量、类别（γ(1)或γ(2)）和亲和力。3. 个体豚鼠的γ(1) -抗体和γ(2) -抗体的亲和力通常非常相似。4. 与电荷较少的γ(2) -抗体的沉淀曲线相比，γ(1) -抗体的沉淀曲线在抗原过量时显示出对沉淀的更显著抑制，表明非特异性作用力在沉淀反应中的重要性。