Ueyama M, Urayama T
Jpn J Exp Med. 1978 Apr;48(2):135-42.
Fibrin stabilizing factor, Factor XIII (F-XIII), which is a transamidating enzyme for fibrin crosslinking, is required for wound healing or fibroblast proliferation. Two know whether or not the enzyme reacts directly to the cell surface, the 3T6 fibroblasts were cultured on fibrin which is natural matrix for the proliferation. Poor-crosslinked and crosslinked fibrin plates were prepared by human plasma clotting with or without the F-XIII inhibitors. In 48 hours culture, the seeded 3 X 10(4) cells were grown to 10.2 X 10(4) on the crosslinked fibrin but only to 3.8 X 10(4) on the poor-crosslinked fibrin plate on the average. The effect of an antifibrinolytic agent, epsilon-aminocaptroic acid was also checked. Consequently, the crosslinking of fibrin seemed to be essential to provide it with favorable property as the contact surface for the growth.
纤维蛋白稳定因子，即因子 XIII（F-XIII），是一种用于纤维蛋白交联的转酰胺酶，在伤口愈合或成纤维细胞增殖中发挥作用。为了明确该酶是否直接作用于细胞表面，将 3T6 成纤维细胞培养在作为增殖天然基质的纤维蛋白上。通过在有或没有 F-XIII 抑制剂的情况下使人血浆凝血，制备交联不良和交联的纤维蛋白平板。在 48 小时的培养中，接种的 3×10⁴ 个细胞在交联纤维蛋白上平均生长至 10.2×10⁴ 个，但在交联不良的纤维蛋白平板上仅生长至 3.8×10⁴ 个。还检测了抗纤溶药物 ε-氨基己酸的作用。因此，纤维蛋白的交联似乎对于赋予其作为生长接触表面的良好特性至关重要。
