一种从牛脊髓中提取胆固醇和磷脂的改进方法。
An improved method for the extraction of cholesterol and phospholipids from the bovine spinal cord.
作者信息
Ali S A, Mahmood I, Khalid Q
出版信息
Prep Biochem. 1978;8(2-3):113-8. doi: 10.1080/00327487808069053.
A pharmacopoeial grade of cholesterol and a separate phospholipid fraction can be obtained by this simple extraction procedure of bovine spinal cord. The tissue is collected in 5% (Wt/V) formalin. It is then separated from the formalin solution, minced and dried in a hot-air oven at 105 degrees C. Total lipids are extracted with petroleum ether. The cholesterol is separated from the phospholipids by extraction with a mixture of petroleum ether and acetone. The method allows for not only the recovery of the phospholipids but also a high yield of cholesterol without using saponification or tedious purification procedures.
通过这种简单的牛脊髓提取程序可以获得药典级胆固醇和单独的磷脂部分。将组织收集在5%（重量/体积）的福尔马林中。然后将其与福尔马林溶液分离，切碎并在105摄氏度的热风烘箱中干燥。用石油醚提取总脂质。通过用石油醚和丙酮的混合物提取，将胆固醇与磷脂分离。该方法不仅可以回收磷脂，还能在不使用皂化或繁琐纯化程序的情况下获得高产率的胆固醇。
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