Klobusická M
Neoplasma. 1976;23(1):11-21.
The effect of GVHR on thymus-dependent lymphocytes of various lymphoid organs has been followed autoradiographically and by scintillation technique in a temporal dependence on the induction of the reaction. GVHR was induced in (BD X Lw) F1 hybrid rats with parental spleen (BD) cells. As marker of the thymus-dependent lymphocytes the in vitro 3H-uridine incorporation was used. Compared to B cells, T lymphocytes of rats have a higher ability of incorporating labeled uridine. T lymphocytes in peripheral blood and the thoracic duct achieved their peak at 3 or 4 days following GVHR induction. On the fourth day, simultaneously with a GVH-altered thymus a rapid decline in the number of these cells was also observed. A mobilization of T lymphocytes from the spleen and lymph nodes into the circulation already in the very first days of the reaction might be responsible for the peak seen in PBL and TDL. The GVH-alteration lasting 21 days caused an evident reduction of thymus-dependent lymphocytes in all the organs studied here. The immunological attack of GVHR is considered to be primarily responsible for the exhaustion of T cells. The discussion bears on the possibility of a more progressive action of GVHR on the more mature T lymphocyte subpopulation.
通过放射自显影术和闪烁技术，在时间上追踪移植物抗宿主反应（GVHR）对各种淋巴器官中胸腺依赖性淋巴细胞的影响，该反应的诱导情况也在时间上进行了追踪。用亲代脾脏（BD）细胞在（BD×Lw）F1杂种大鼠中诱导GVHR。使用体外3H-尿苷掺入作为胸腺依赖性淋巴细胞的标志物。与B细胞相比，大鼠的T淋巴细胞具有更高的掺入标记尿苷的能力。外周血和胸导管中的T淋巴细胞在GVHR诱导后3或4天达到峰值。在第四天，与发生GVH改变的胸腺同时，还观察到这些细胞数量迅速下降。在反应的最初几天，T淋巴细胞从脾脏和淋巴结动员到循环中可能是外周血淋巴细胞（PBL）和胸导管淋巴细胞（TDL）中出现峰值的原因。持续21天的GVH改变导致此处研究的所有器官中的胸腺依赖性淋巴细胞明显减少。GVHR的免疫攻击被认为是T细胞耗竭的主要原因。讨论涉及GVHR对更成熟的T淋巴细胞亚群可能具有更渐进作用的可能性。
