Heby O, Emanuelsson H
Cell Tissue Res. 1978 Nov 9;194(1):103-14. doi: 10.1007/BF00209236.
Continuous treatment of developing eggs of the polychete Ophryotrocha labronica with alpha-methylornithine, which inhibits synthesis of putrescine, led to arrest of development at gastrulation. The present ultrastructural analysis suggests that the arrest of development is due to failure to form nuclei, and thus reveals a possible role for putrescine in nucleolar formation. Further support for this contention was provided by means of electron-microscopical autoradiography. It was found that newly synthesized putrescine, derived from administered 3H-ornithine, labeled the nucleoli intensely at the time of their normal appearance during gastrulation, the time at which the rate of endogenous putrescine synthesis is maximal. These observations have led to the conclusion that putrescine synthesis may be directly involved in formation of nucleoli.
用抑制腐胺合成的α-甲基鸟氨酸持续处理多毛纲动物拉氏角管虫发育中的卵，会导致发育在原肠胚形成阶段停止。目前的超微结构分析表明，发育停止是由于未能形成细胞核，从而揭示了腐胺在核仁形成中可能发挥的作用。电子显微镜放射自显影法为这一论点提供了进一步支持。研究发现，由施用的3H-鸟氨酸衍生而来的新合成腐胺，在原肠胚形成期间核仁正常出现时（即内源性腐胺合成速率最高的时候）强烈标记核仁。这些观察结果得出结论，腐胺合成可能直接参与核仁的形成。
