Freudenstein C, Franke W W, Osborn M, Weber K
Cell Biol Int Rep. 1978 Nov;2(6):591-600. doi: 10.1016/0309-1651(78)90068-1.
Total purified and reconstituted bovine hoof prekeratin, containing several polypeptides, as well as individual polypeptide size classes isolated therefrom were used as antigens in guinea pigs. The antibodies raised against these protein preparations were found to decorate the system of wavy arrays of tonofilament-like, intermediate-sized filaments present in various epithelial and epithelia-derived cells. Strong cross-reaction between different vertebrate species was noted, including amphibia. Positive results were obtained with original sera as well as with IgG fractions and antibodies made monospecific by chromatography on total bovine prekeratin covalently bound to Sepharose. Among the antisera raised against the different polypeptide size classes the most intense decoration of fibrillar arrays was obtained with antibodies against fraction 4 which contained polypeptides VI and VII.
含有几种多肽的总纯化和重组牛蹄前角蛋白，以及从中分离出的各个多肽大小类别，被用作豚鼠的抗原。发现针对这些蛋白质制剂产生的抗体能够修饰存在于各种上皮细胞和上皮衍生细胞中的丝状、中等大小的丝状纤维波浪状排列系统。注意到不同脊椎动物物种之间有强烈的交叉反应，包括两栖动物。原始血清、IgG 组分以及通过在共价结合到琼脂糖的总牛前角蛋白上进行色谱法制备的单特异性抗体都获得了阳性结果。在针对不同多肽大小类别的抗血清中，针对含有多肽 VI 和 VII 的第 4 组分的抗体对纤维状阵列的修饰最为强烈。
