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Cloning of cDNA and amino acid sequence of a cytokeratin expressed in oocytes of Xenopus laevis.

作者信息

Franz J K, Franke W W

出版信息

Proc Natl Acad Sci U S A. 1986 Sep;83(17):6475-9. doi: 10.1073/pnas.83.17.6475.

DOI:10.1073/pnas.83.17.6475
PMID:2428034
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC386526/
Abstract

Using a cDNA clone from the ovary of the frog, Xenopus laevis, we have identified the mRNA and determined the complete amino acid sequence of a major cytoskeletal protein expressed in the oocyte. A comparison with other cytoskeletal proteins of Xenopus and mammals identifies this polypeptide Mr 55,700 as a nonepidermal kind of cytokeratin of the basic (type II) subfamily, which represents the amphibian equivalent to cytokeratin no. 8 of simple epithelia of higher mammals. The sequence data demonstrate the high evolutionary stability of this protein. This cytokeratin and its mRNA are present in oocytes, eggs, embryos, liver, and intestinal mucosa of adult frogs, as well as cultured kidney epithelial cells. We suggest that epithelial cell differentiation in early stages of Xenopus embryogenesis differs from other known pathways of cell differentiation in that major cell-type-specific proteins--i.e., cytokeratins of the simple epithelial type--and their mRNAs are maternally provided and distributed to early epithelial cells by special sorting mechanisms.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bae3/386526/f18fb9710d07/pnas00321-0253-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bae3/386526/6c129e169954/pnas00321-0252-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bae3/386526/f18fb9710d07/pnas00321-0253-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bae3/386526/6c129e169954/pnas00321-0252-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bae3/386526/f18fb9710d07/pnas00321-0253-a.jpg

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1
Cloning of cDNA and amino acid sequence of a cytokeratin expressed in oocytes of Xenopus laevis.
Proc Natl Acad Sci U S A. 1986 Sep;83(17):6475-9. doi: 10.1073/pnas.83.17.6475.
2
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4
Amino acid sequence of the carboxy-terminal part of an acidic type I cytokeratin of molecular weight 51 000 from Xenopus laevis epidermis as predicted from the cDNA sequence.根据cDNA序列预测的来自非洲爪蟾表皮的分子量为51000的酸性I型细胞角蛋白羧基末端部分的氨基酸序列。
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5
Developmentally regulated cytokeratin gene in Xenopus laevis.非洲爪蟾中受发育调控的细胞角蛋白基因。
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Cytokeratin expression in simple epithelia. I. Identification of mRNA coding for human cytokeratin no. 18 by a cDNA clone.细胞角蛋白在单层上皮中的表达。I. 利用cDNA克隆鉴定编码人细胞角蛋白18号的mRNA
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本文引用的文献

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Translational products of mRNAs coding for non-epidermal cytokeratins.编码非表皮细胞角蛋白的信使核糖核酸的翻译产物。
EMBO J. 1983;2(8):1387-92. doi: 10.1002/j.1460-2075.1983.tb01596.x.
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A major developmental transition in early Xenopus embryos: II. Control of the onset of transcription.非洲爪蟾早期胚胎中的一个主要发育转变：II. 转录起始的控制
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简单、分层、角化和角质化上皮中角蛋白蛋白的结构和功能。
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In Vitro Cell Dev Biol Anim. 2003 Jan-Feb;39(1-2):29-35. doi: 10.1290/1543-706X(2003)039<0029:COAWBM>2.0.CO;2.
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A nontetrameric species is the major soluble form of keratin in Xenopus oocytes and rabbit reticulocyte lysates.非四聚体形式是非洲爪蟾卵母细胞和兔网织红细胞裂解物中角蛋白的主要可溶性形式。
J Cell Biol. 1996 Jan;132(1-2):153-65. doi: 10.1083/jcb.132.1.153.
7
Bovine filensin possesses primary and secondary structure similarity to intermediate filament proteins.牛丝状肌动蛋白与中间丝蛋白在一级和二级结构上具有相似性。
J Cell Biol. 1993 May;121(4):847-53. doi: 10.1083/jcb.121.4.847.
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Genomic rearrangements of retroviral vectors carrying two genes in F9 EC cells.F9胚胎癌细胞中携带两个基因的逆转录病毒载体的基因组重排。
Virus Genes. 1993 Feb;7(1):53-65. doi: 10.1007/BF01702348.
9
The 47-kD lens-specific protein phakinin is a tailless intermediate filament protein and an assembly partner of filensin.47-kD晶状体特异性蛋白phakinin是一种无尾中间丝蛋白，也是丝状晶状体蛋白的组装伙伴。
J Cell Biol. 1993 Dec;123(6 Pt 1):1507-16. doi: 10.1083/jcb.123.6.1507.
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Molecular characterization of a karyophilic, histone-binding protein: cDNA cloning, amino acid sequence and expression of nuclear protein N1/N2 of Xenopus laevis.一种亲核组蛋白结合蛋白的分子特征：非洲爪蟾核蛋白N1/N2的cDNA克隆、氨基酸序列及表达
EMBO J. 1986 Dec 20;5(13):3547-52. doi: 10.1002/j.1460-2075.1986.tb04681.x.
Cold Spring Harb Symp Quant Biol. 1982;46 Pt 1:431-53. doi: 10.1101/sqb.1982.046.01.041.
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Formation of cytoskeletal elements during mouse embryogenesis. II. Epithelial differentiation and intermediate-sized filaments in early postimplantation embryos.小鼠胚胎发育过程中细胞骨架成分的形成。II. 植入后早期胚胎中的上皮分化和中间丝
Differentiation. 1981;20(3):203-16. doi: 10.1111/j.1432-0436.1981.tb01177.x.
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Monoclonal antibodies against trophectoderm-specific markers during mouse blastocyst formation.针对小鼠囊胚形成过程中滋养外胚层特异性标志物的单克隆抗体。
Proc Natl Acad Sci U S A. 1980 Jul;77(7):4113-7. doi: 10.1073/pnas.77.7.4113.
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Differential gene expression in the gastrula of Xenopus laevis.非洲爪蟾原肠胚中的差异基因表达。
Science. 1983 Oct 14;222(4620):135-9. doi: 10.1126/science.6688681.
7
Cell type-specific activation of actin genes in the early amphibian embryo.早期两栖动物胚胎中肌动蛋白基因的细胞类型特异性激活。
Nature. 1984;311(5988):716-21. doi: 10.1038/311716a0.
8
Transcription of muscle-specific actin genes in early Xenopus development: nuclear transplantation and cell dissociation.非洲爪蟾早期发育中肌肉特异性肌动蛋白基因的转录：核移植与细胞解离
Cell. 1984 Oct;38(3):691-700. doi: 10.1016/0092-8674(84)90264-2.
9
Oocytes and early embryos of Xenopus laevis contain intermediate filaments which react with anti-mammalian vimentin antibodies.非洲爪蟾的卵母细胞和早期胚胎含有能与抗哺乳动物波形蛋白抗体发生反应的中间丝。
J Embryol Exp Morphol. 1984 Oct;83:169-87.
10
Intermediate (10 nm) filament proteins and the Ca2+-activated proteinase specific for vimentin and desmin in the cells from fish to man: an example of evolutionary conservation.从鱼类到人类细胞中的中间丝（10纳米）蛋白以及对波形蛋白和结蛋白具有特异性的钙激活蛋白酶：进化保守性的一个实例。
J Cell Sci. 1982 Oct;57:25-49. doi: 10.1242/jcs.57.1.25.