单克隆抗体为上皮张力丝组织的研究提供了特异性的分子内标记。
Monoclonal antibodies provide specific intramolecular markers for the study of epithelial tonofilament organization.
作者信息
Lane E B
出版信息
J Cell Biol. 1982 Mar;92(3):665-73. doi: 10.1083/jcb.92.3.665.
Abstract
The tonofilament-associated protein antigens recognized in epithelial cells by a group of six monoclonal antibodies have been studied by immunofluorescence and gel immunoautoradiography. The monoclonal antibodies were generated against detergent insoluble cytoskeleton extracts from a cultured simple epithelium derived cell line, Ptk1 cells. They show various tissue specificities, and while they all recognize components at the low end of the molecular weight range for intermediate filament proteins, they confirm that single antibody species can react with multiple polypeptides of different molecular weights in the tonofilament complex. The monoclonal antibodies described here demonstrate the presence of a simple epithelium antigenic determinant associated with intermediate filaments that is not detectable in the specialized cells of squamous and keratinizing epithelia but can reappear in such cells after transformation.
摘要
通过免疫荧光和凝胶免疫放射自显影技术，对一组六种单克隆抗体在上皮细胞中识别的张力丝相关蛋白抗原进行了研究。这些单克隆抗体是针对从培养的简单上皮来源细胞系Ptk1细胞的去污剂不溶性细胞骨架提取物产生的。它们表现出不同的组织特异性，虽然它们都识别中间丝蛋白分子量范围低端的成分，但证实单个抗体种类可与张力丝复合物中不同分子量的多种多肽发生反应。本文所述的单克隆抗体证明了与中间丝相关的简单上皮抗原决定簇的存在，该决定簇在鳞状和角质化上皮的特化细胞中无法检测到，但在转化后可在这类细胞中重新出现。
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