Williams G M, Bermudez E, San R H, Goldblatt P J, Laspia M F
In Vitro. 1978 Oct;14(10):824-37. doi: 10.1007/BF02616152.
Primary cultures of rat hepatocytes survived well for up to 4 days in defined medium in the presence of dexamethasone but not in its absence. The loss of viability was accompanied by a loss of ultrastructural features characteristic of hepatocytes. The cultures began producing plasminogen activator and a neutral protease after 24 hr in culture. Dexamethasone inhibited the production of both of these substances. The deterioration of the cultures appeared not to be related to plasminogen activator, but prolongation of survival by a variety of protease inhibitors suggested that the neutral protease might contribute to deterioration.
在含有地塞米松的限定培养基中，大鼠肝细胞原代培养物在长达4天的时间里存活良好，但在没有地塞米松的情况下则不然。活力丧失伴随着肝细胞特征性超微结构特征的丧失。培养24小时后，培养物开始产生纤溶酶原激活物和一种中性蛋白酶。地塞米松抑制这两种物质的产生。培养物的恶化似乎与纤溶酶原激活物无关，但多种蛋白酶抑制剂延长了存活时间，这表明中性蛋白酶可能导致了恶化。
