Burroughs J N, Brown F
J Gen Virol. 1978 Nov;41(2):443-6. doi: 10.1099/0022-1317-41-2-443.
The infective RNA of the calicivirus, vesicular exanthema virus, has been shown to contain a protein which is apparently linked to the RNA by a covalent bond. The protein remained bound to the RNA after boiling with SDS-mercaptoethanol-urea or treating with formamide-dimethylsulphoxide but was removed by incubating with proteinase K. The mol. wt. of the protein was estimated to be about 1o X 1O(3) by electrophoresis in highly cross-linked polyacrylamide gels. The infectivity of the RNA was destroyed by removal of the protein with proteinase K.
杯状病毒——水疱性口炎病毒的感染性RNA已被证明含有一种蛋白质，该蛋白质显然通过共价键与RNA相连。在用SDS - 巯基乙醇 - 尿素煮沸或用甲酰胺 - 二甲基亚砜处理后，该蛋白质仍与RNA结合，但用蛋白酶K孵育可将其去除。通过在高度交联的聚丙烯酰胺凝胶中电泳，估计该蛋白质的分子量约为10×10³。用蛋白酶K去除蛋白质会破坏RNA的感染性。
