Burroughs J N, Doel T R, Smale C J, Brown F
J Gen Virol. 1978 Jul;40(1):161-74. doi: 10.1099/0022-1317-40-1-161.
The structure of vesicular exanthema virus, the prototype member of the calicivirus group, has been studied in more detail. The RNA comprises 18% of mol. wt. of about 2.8 x 10(6), based on polyacrylamide gel electrophoresis experiments in the presence of formaldehyde. The virus contains one major polypeptide, mol. wt. 70 x 10(3) as determined by polyacrylamide gel electrophoresis and by chromatography on Sepharose 6B in the presence of 6 M-guanidine. Further evidence for the presence of a single major polypeptide was obtained by tryptic peptide analysis of 35S-methionine labelled virus. The mol. wt. of a protein oligomer produced by adjusting the pH of virus suspensions to 3.5 was c. 200 x 10(3). On the basis of these data we propose a T = 3 model for the virus capsid incorporating 180 copies of the virus protein.
杯状病毒组的原型成员——水疱性口炎病毒的结构已得到更详细的研究。根据在甲醛存在下进行的聚丙烯酰胺凝胶电泳实验，RNA占分子量约2.8×10⁶的18%。该病毒含有一种主要多肽，通过聚丙烯酰胺凝胶电泳以及在6M胍存在下于琼脂糖6B上进行色谱分析确定其分子量为70×10³。通过对³⁵S - 甲硫氨酸标记病毒进行胰蛋白酶肽分析，获得了存在单一主要多肽的进一步证据。通过将病毒悬液的pH值调至3.5产生的一种蛋白质寡聚体的分子量约为200×10³。基于这些数据，我们提出了一种病毒衣壳的T = 3模型，该模型包含180个病毒蛋白拷贝。
