Benedetti E L, Emmelot P
J Cell Biol. 1968 Jul;38(1):15-24. doi: 10.1083/jcb.38.1.15.
Solubilization of isolated rat liver plasma membranes in 1% deoxycholate and centrifugation yielded a fraction (pellet) that consisted mainly of tight junctions (zonulae occludentes). An hexagonal array of subunits similar to that previously found in a number of the unfractionated plasma membranes was demonstrated in all the membrane sheets of these preparations by negative staining. It is concluded that the hexagonal subunit pattern is present in the tight junctions, and that this structural differentiation may be related to the intercellular diffusion afforded by the junctional membrane.
将分离的大鼠肝细胞膜在1%脱氧胆酸盐中溶解并离心，得到一个主要由紧密连接（闭锁小带）组成的组分（沉淀）。通过负染色在这些制剂的所有膜片中都显示出一种类似于先前在许多未分级的质膜中发现的六边形亚基阵列。得出的结论是，紧密连接中存在六边形亚基模式，并且这种结构分化可能与连接膜提供的细胞间扩散有关。
