辛德毕斯病毒突变体。III. 纯化的HR和ts103病毒颗粒中存在的宿主多肽。
Mutants of Sindbis virus. III. Host polypeptides present in purified HR and ts103 virus particles.
作者信息
Strauss E G
出版信息
J Virol. 1978 Nov;28(2):466-74. doi: 10.1128/JVI.28.2.466-474.1978.
Abstract
The amounts of host-encoded protein present in purified Sindbis virions of both the HR strain and of a mutant (ts103) which makes multicored particles were examined. Cells were labeled with [35S]methionine before infection and with [3H]methionine postinfection. Virions were purified by velocity sedimentation and isopycnic banding, and their polypeptides were examined by polyacrylamide gels in a sodium dodecyl sulfate-containing discontinuous buffer system. Host prelabeled material was found principally in a small number of discrete polypeptides in HR virions, which contained as little as 0.2% host-encoded protein. Virus-sized particles of mutant ts103 contained significantly more host material, and multiploid particles from ts103 infection contained up to 12% host prelabeled protein.
摘要
对HR株和产生多核颗粒的突变体（ts103）的纯化辛德毕斯病毒粒子中宿主编码蛋白的含量进行了检测。感染前用[35S]甲硫氨酸标记细胞，感染后用[3H]甲硫氨酸标记。通过速度沉降和等密度梯度离心法纯化病毒粒子，并在含十二烷基硫酸钠的不连续缓冲系统中通过聚丙烯酰胺凝胶对其多肽进行检测。在HR病毒粒子中，宿主预标记物质主要存在于少数离散的多肽中，这些多肽所含的宿主编码蛋白低至0.2%。突变体ts103的病毒大小颗粒含有明显更多的宿主物质，ts103感染产生的多倍体颗粒含有高达12%的宿主预标记蛋白。
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