大鼠肝脏质膜组分对溶血磷脂的酰化作用。
Acylation of lysophosphatides by plasma membrane fractions of rat liver.
作者信息
Stein Y, Widnell C, Stein O
出版信息
J Cell Biol. 1968 Oct;39(1):185-92. doi: 10.1083/jcb.39.1.185.
Abstract
The plasma membrane fraction of rat liver was isolated and incubated with labeled lysophosphatides in the presence of cofactors; the acylation of lysolecithin to lecithin by the fraction was compared to that of the rough and smooth microsomes. The purity of the isolated fractions was ascertained by enzyme markers and electron microscopy, and the maximal contamination of the plasma membrane fraction by microsomes did not exceed 20%. Under conditions at which the reaction was proportional to the amount of enzyme used, the plasma membrane had a specific activity similar to that of the smooth and rough microsomes. With doubly labeled lysolecithin (containing palmitic acid-(14)C and choline-(3)H) it was shown that the lecithin formed retained the same ratio of the two labels, which indicated that lysolecithin was converted to lecithin through an acylation reaction. The newly formed lecithin was shown to be bound to the plasma membrane fraction; this suggested that it is incorporated into the structure of the membrane itself.
摘要
分离大鼠肝脏的质膜部分，并在辅因子存在的情况下与标记的溶血磷脂一起孵育；将该部分将溶血卵磷脂酰化为卵磷脂的能力与粗面和滑面微粒体的能力进行比较。通过酶标记和电子显微镜确定分离部分的纯度，质膜部分被微粒体的最大污染不超过20%。在反应与所用酶量成比例的条件下，质膜具有与滑面和粗面微粒体相似的比活性。用双标记的溶血卵磷脂（含有棕榈酸 -（14）C和胆碱 -（3）H）表明，形成的卵磷脂保留了两种标记的相同比例，这表明溶血卵磷脂通过酰化反应转化为卵磷脂。新形成的卵磷脂被证明与质膜部分结合；这表明它被整合到膜本身的结构中。
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