[从无定形和结晶核糖核酸酶生产核糖核酸酶A的改进方法]

[Modified method for producing ribonuclease A from amorphous and crystalline ribonucleases].

作者信息

Lebedev Iu O, Shliapnikov S V, Borisova S N, Abaturov L V

出版信息

Prikl Biokhim Mikrobiol. 1981 Mar-Apr;17(2):247-53.

Abstract

The paper describes a method for producing gram-scale quantities of beef pancreatic ribonuclease A (RNase A) (EC 3.1.4.22) from commercial, amorphous and crystalline RNases. The method involves enzyme purification by chromatography on SP-Sephadex G-25, rechromatography on Bio-Rax 70 followed by concentration, storage and desalting in optimal conditions. In their properties the resultant preparations of RNase A are not inferior to the best commercial samples of the enzyme.

摘要

本文描述了一种从市售的无定形和结晶核糖核酸酶中生产克级量牛胰核糖核酸酶A（RNase A）（EC 3.1.4.22）的方法。该方法包括通过在SP-葡聚糖凝胶G-25上进行色谱法纯化酶，然后在Bio-Rax 70上进行再色谱法，接着在最佳条件下进行浓缩、储存和脱盐。所得的RNase A制剂在性质上不逊色于该酶的最佳市售样品。