Stoianov T, Kostadinov B
Vet Med Nauki. 1978;15(3):47-54.
A new medium was produced, containing no glycerin but rich in raffinose, egg yolk, and mineral water, adequate for freezing bull semen. It was tested parallel to a control extender, consisting of yolk, lactose, and glycerine. The absolute survival rate of the spermatozoa kept at 0-4 degrees C in a raffinose medium proved by 24.25 h higher than that of the spermatozoa in the control medium. No other essential differences were established as to motility, heat resistance and percent of pathologic forms of freshly diluted and frozen semen between the test and the control medium. The biologic test on a total of 133 cows resulted in a 71.4% conception rate at first insemination. The semen frozen in the control medium showed 62.6% conception at the insemination of 123 cows. The difference of 8.8% was in favour of the new synthetic medium. The insufficient number of animals (133) used in the investigations made it necessary to test the new medium on a wider production basis.
一种新的培养基被研制出来，其不含甘油，但富含棉子糖、蛋黄和矿泉水，适用于冷冻公牛精液。它与一种由蛋黄、乳糖和甘油组成的对照稀释液平行进行测试。在棉子糖培养基中于0 - 4摄氏度保存的精子的绝对存活率经证明比对照培养基中的精子高24.25小时。在新鲜稀释和冷冻精液的活力、耐热性及病理形态百分比方面，测试培养基和对照培养基之间未发现其他本质差异。对总共133头母牛进行的生物学测试表明，首次输精时受孕率为71.4%。在对照培养基中冷冻的精液在给123头母牛输精时受孕率为62.6%。8.8%的差异有利于这种新的合成培养基。研究中使用的动物数量不足（133头），因此有必要在更广泛的生产基础上对这种新培养基进行测试。
