Stoianov T, Kostadinov B
Vet Med Nauki. 1975;12(10):3-9.
Semen was used of four breeding bulls of the Bulgarian Red breed and the Alpine Brown one. A total of 26 ejaculates were subjected to 344 laboratory investigations. The semen was diluted in a yolk-lactose-glycerine medium and was frozen by the fast method of Nagase-Niwa. Studied were in dynamics some biologic and biochemical indexes, such as: heat resistance at 46 degrees C, percentage of dead and pathologic spermatozoa, intake of oxygen, and release of CO2. The respiratory coefficient was established by the direct method of Umbreit and coll. The survival rate proved better, lower was the number of dead and pathologic spermatozoa in a test medium containing slightly mineralized water. The consumption of oxygen and the amount of the CO2 released by spermatozoa showed a dependable decrease following equilibration and freezing. Similar, however, unreliable were the data of changes observed with the respiratory coefficient. The semen frozen in the new synthetic medium showed higher biologic value and higher fertilizing capacity (12.2 per cent) as compared with the control.
使用了4头保加利亚红牛品种和阿尔卑斯棕牛品种的种公牛的精液。总共26份射精样本接受了344次实验室检测。精液在蛋黄 - 乳糖 - 甘油培养基中稀释，并采用长谷川 - 丹羽快速冷冻法进行冷冻。对一些生物学和生化指标进行了动态研究，如：46摄氏度时的耐热性、死精和病理精子的百分比、氧气摄入量以及二氧化碳释放量。呼吸系数采用翁布雷特等人的直接法测定。结果表明，在含有微矿化水的测试培养基中，精子的存活率更高，死精和病理精子的数量更少。精子平衡和冷冻后，氧气消耗量和二氧化碳释放量可靠地减少。然而，呼吸系数变化的数据却不可靠。与对照相比，在新合成培养基中冷冻的精液显示出更高的生物学价值和更高的受精能力（12.2%）。
