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[用于分离“逆转录酶”的禽成髓细胞瘤病毒繁殖条件的研究]

[Study of the reproduction conditions of the avian myeloblastosis virus for separation of the "reverse transcriptase" enzyme].

作者信息

Graevskaia N A, Sito A F, Dumina A L

出版信息

Biull Eksp Biol Med. 1975 Jun;79(6):56-9.

PMID:56958
Abstract

Sensitivity of eight chick lines to the avian myeloblastosis virus as the main source for RNA-dependent DNA-polymerase recovery was studied in the course of the "revertase" project. The virus (0.1 ml) was inoculated intracardially or intraperitoneally to one-day chicks, and then the virus titer was determined according to the ATP-activity. C and D-lines of the Enya-cross were shown to be most sensitive (sensitivity 75%) among the lines studied.

摘要

在“逆转酶”项目过程中,研究了八个鸡品系对禽成髓细胞瘤病毒的敏感性,该病毒是恢复依赖RNA的DNA聚合酶的主要来源。将病毒(0.1毫升)经心内或腹腔接种给一日龄雏鸡,然后根据ATP活性测定病毒滴度。结果显示,在所研究的品系中,恩亚杂交系的C和D品系最为敏感(敏感性为75%)。

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