Thompson J N, Madère R
J Assoc Off Anal Chem. 1978 Nov;61(6):1370-3.
A previously published fluorometric method for vitamin A in milk, involving saponification in centrifuge tubes and extraction with hexane, was automated using conventional automated equipment including a filter fluorometer. The extraction and separation steps were affected by the presence of fat, so standards were prepared in milk. The peaks obtained with samples were 95% of steady state values. The coefficients of variation for 10 replicate analyses were 1.9% for unfortified milk and 1.3% for fortified milk. In tests on 19 different milk samples, the results of the automated and manual fluorometric methods differed by less than 10%; a colorimetric method using SbCl3 gave more erratic results and was more lengthy and laborious.
一种先前发表的用于测定牛奶中维生素A的荧光法，该方法涉及在离心管中进行皂化并用己烷萃取，现使用包括滤光荧光计在内的常规自动化设备实现了自动化。萃取和分离步骤受到脂肪存在的影响，因此在牛奶中制备标准品。样品获得的峰为稳态值的95%。10次重复分析的变异系数，未强化牛奶为1.9%，强化牛奶为1.3%。在对19个不同牛奶样品的测试中，自动化荧光法和手动荧光法的结果差异小于10%；使用SbCl3的比色法结果更不稳定，且耗时更长、操作更繁琐。
