Simmons N A
J Clin Pathol. 1968 Mar;21(2):196-201. doi: 10.1136/jcp.21.2.196.
A method for the determination of both direct and indirect serum bilirubin on the AutoAnalyzer is described which has a number of advantages over the automated method that is currently recommended. It gives total bilirubin results which are similar to those obtained with the equivalent manual method and direct bilirubin results which are usually about 10% lower.The effect of haemolysis on estimations was investigated. It has little influence on the total, but significantly lowers the direct bilirubin results. Simple methods for the preparation of true bilirubin standards and a stable control serum are described.
本文描述了一种在自动分析仪上测定血清直接胆红素和间接胆红素的方法，该方法与目前推荐的自动化方法相比具有许多优点。它给出的总胆红素结果与等效手工方法得到的结果相似，而直接胆红素结果通常低约10%。研究了溶血对测定的影响。溶血对总胆红素影响不大，但会显著降低直接胆红素结果。文中还描述了制备真实胆红素标准品和稳定对照血清的简单方法。
