Cummings D J, Chapman V A, DeLong S S
J Virol. 1968 Jun;2(6):610-20. doi: 10.1128/JVI.2.6.610-620.1968.
Dimethyl sulfoxide (DMSO) disrupted T-even bacteriophages as well as lambda bacteriophage. The component substructures of T2L, T4B01, or T6, in particular heads, were readily isolated after treatment with 67% DMSO (v/v). In contrast, concentrations of DMSO above 50% not only separated heads from tails of bacteriophage lambda but led to degradation of the lambda heads. Examination of the isolated free heads of T-even bacteriophage indicated that a distinct neck substructure was attached to one apex of the head. On some free tails a similar neck substructure was also found at the proximal end of the sheath. The dimensions of this neck substructure were found to be about 130 by 180 A; by virtue of its size and morphological attachment to the free heads, it was concluded that this was a distinct substructure and not an extension of the tail tube.
二甲基亚砜（DMSO）会破坏T偶数噬菌体以及λ噬菌体。在用67%（体积/体积）的DMSO处理后，T2L、T4B01或T6的组成子结构，特别是头部，很容易被分离出来。相比之下，高于50%的DMSO浓度不仅会使λ噬菌体的头部与尾部分离，还会导致λ噬菌体头部的降解。对T偶数噬菌体分离出的游离头部的检查表明，一个独特 的颈部子结构附着在头部的一个顶点上。在一些游离的尾部，在鞘的近端也发现了类似的颈部子结构。发现这个颈部子结构的尺寸约为130×180埃；由于其大小以及与游离头部的形态学附着关系，可以得出结论，这是一个独特的子结构，而不是尾管的延伸。
