Ingle J
Plant Physiol. 1968 Nov;43(11):1850-4. doi: 10.1104/pp.43.11.1850.
Chloroplast RNA is synthesized in dark-grown radish cotyledons at about one-third the rate of that in the light. The synthesis, however, continues for longer in the dark and the percentage of chloroplast RNA can approach that in light-grown tissue. Light stimulates the synthesis and accumulation of both cytoplasmic and chloroplast RNA, but shows a 4-fold greater stimulation of the chloroplast RNA. Chloramphenicol, streptomycin and cycloheximide inhibit the synthesis of chloroplast RNA with little effect on cytoplasmic RNA. 5-Fluorouracil inhibits the synthesis of cytoplasmic more than chloroplast RNA. Synthesis of the 0.56 x 10(6) mol wt chloroplast RNA is inhibited much less than the other ribosomal RNA components by actinomycin D.
叶绿体RNA在黑暗中生长的萝卜子叶中的合成速率约为光照下的三分之一。然而，这种合成在黑暗中持续的时间更长，叶绿体RNA的百分比可以接近光照下生长的组织中的百分比。光照刺激细胞质和叶绿体RNA的合成与积累，但对叶绿体RNA的刺激作用要大4倍。氯霉素、链霉素和环己酰亚胺抑制叶绿体RNA的合成，对细胞质RNA影响很小。5-氟尿嘧啶对细胞质RNA合成的抑制作用比对叶绿体RNA的抑制作用更大。放线菌素D对0.56×10⁶摩尔重量的叶绿体RNA合成的抑制作用远小于对其他核糖体RNA组分的抑制作用。
