Pitha J
Cancer Res. 1976 Apr;36(4):1273-7.
The effects of poly(1-vinyluracil) [poly(vU)] and poly(9-vinyladenine) [poly(vA)] on the RNA-dependent DNA polymerase activity of murine leukemia virus (Moloney strain) were studied. Vinyl polymers themselves cannot act as templates for the polymerase. However, if a vinyl polymer is added to a polymerase reaction mixture in which a complementary polynucleotide serves as the template, the reaction is inhibited: thus with polyribocytidylic acid as template and oligodeoxyguanylic acid as primer, neither poly(vU) nor poly(vA) had a significant effect; when polyribouridylic acid was used as template and oligodeoxyadenylic acid as primer, poly(vA) inhibited polymerase activity while poly(vU) had little effect; when polyriboadenylic acid was a template and oligodeoxy thymidylic acid was a primer, poly(vU) was an inhibitor. Complex effects were noted with the latter system and poly(vA); either stimulation or inhibition of the reaction was observed, depending on the concentration of poly(vA). The stimulation brings about a decrease in the amount of lower-molecular-weight materials in the product and is caused by the interaction of poly(vA) with the template-primer. Thus vinyl polymers differ from polynucleotides in their mechanism of inhibition of viral polymerase, since the latter inhibit the enzyme by binding to it.
研究了聚（1-乙烯基尿嘧啶）[聚（vU）]和聚（9-乙烯基腺嘌呤）[聚（vA）]对鼠白血病病毒（莫洛尼株）的RNA依赖性DNA聚合酶活性的影响。乙烯基聚合物本身不能作为聚合酶的模板。然而，如果将乙烯基聚合物添加到以互补多核苷酸为模板的聚合酶反应混合物中，反应会受到抑制：因此，以聚核糖胞苷酸为模板、寡聚脱氧鸟苷酸为引物时，聚（vU）和聚（vA）均无显著影响；当以聚核糖尿苷酸为模板、寡聚脱氧腺苷酸为引物时，聚（vA）抑制聚合酶活性，而聚（vU）影响很小；当以聚核糖腺苷酸为模板、寡聚脱氧胸苷酸为引物时，聚（vU）是一种抑制剂。在后者的体系和聚（vA）中观察到了复杂的效应；根据聚（vA）的浓度，观察到反应的刺激或抑制。刺激导致产物中低分子量物质的量减少，这是由聚（vA）与模板-引物的相互作用引起的。因此，乙烯基聚合物在抑制病毒聚合酶的机制上与多核苷酸不同，因为后者通过与酶结合来抑制酶。
