大肠杆菌RNA聚合酶的模板特异性抑制剂。

Template specific inhibitors of E. coli RNA polymerase.

作者信息

Chou H J, Froehlich J P, Pitha J

出版信息

Nucleic Acids Res. 1978 Mar;5(3):691-6. doi: 10.1093/nar/5.3.691.

DOI:10.1093/nar/5.3.691

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342016/

Abstract

Electroneutral analogs of polynucleotides, poly-9-vinyladenine and poly-1-vinyluracil inhibit E. coli RNA polymerase in all combinations where the single stranded polynucleotides are used as templates and the vinyl analogs are complementary to them. As templates both the ribo- and deoxyribopolynucleotides were tested; variation of the template concentration in the presence of vinyl analogs produced a competitive pattern of inhibition. The electroneutral analogs do not inhibit the enzyme activity when non-complementary single stranded polynucleotides or double stranded polynucleotides are used as templates; the latter fully supports transcription even when one of the strands is complementary to the analog.

摘要

多核苷酸的电中性类似物，聚-9-乙烯基腺嘌呤和聚-1-乙烯基尿嘧啶，在以单链多核苷酸为模板且乙烯基类似物与之互补的所有组合中，均能抑制大肠杆菌RNA聚合酶。作为模板，对核糖和脱氧核糖多核苷酸都进行了测试；在乙烯基类似物存在的情况下，模板浓度的变化产生了竞争性抑制模式。当使用非互补单链多核苷酸或双链多核苷酸作为模板时，电中性类似物不会抑制酶活性；即使其中一条链与类似物互补，双链多核苷酸仍能充分支持转录。

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本文引用的文献

1

Polynucleotide analogue inhibition of messenger-stimulated transfer RNA binding to ribosomes.多核苷酸类似物对信使刺激的转运RNA与核糖体结合的抑制作用。

Biochim Biophys Acta. 1971 Dec 30;254(3):452-6. doi: 10.1016/0005-2787(71)90879-3.

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A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.一种大规模纯化大肠杆菌脱氧核糖核酸依赖性核糖核酸聚合酶的新方法。

J Biol Chem. 1969 Nov 25;244(22):6160-7.

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Inhibition of cell-free protein synthesis by poly(9-vinyladenine), poly(1-vinyluracil), and the corresponding vinyl copolymer.

Biochemistry. 1972 Aug 15;11(17):3261-6. doi: 10.1021/bi00767a021.

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Inhibition of RNA-dependent DNA polymerase of avian myeloblastosis virus by pyran copolymer.吡喃共聚物对禽成髓细胞瘤病毒的RNA依赖性DNA聚合酶的抑制作用

Proc Natl Acad Sci U S A. 1974 Feb;71(2):367-70. doi: 10.1073/pnas.71.2.367.

5

Effects of poly(9-vinyladenine) and poly(1-vinyluracil) on messenger ribonucleic acid template activity.聚（9-乙烯基腺嘌呤）和聚（1-乙烯基尿嘧啶）对信使核糖核酸模板活性的影响。

Mol Pharmacol. 1975 Nov;11(6):708-15.

6

Synthetic analogues of polynucleotides. (Part) XIV. The synthesis of poly (3'-0-carboxymethyl-2'-deoxycytidine) and its interaction with polyinosinic acid.多核苷酸的合成类似物。（第十四部分）。聚（3'-O-羧甲基-2'-脱氧胞苷）的合成及其与聚肌苷酸的相互作用。

Nucleic Acids Res. 1975 May;2(5):699-706. doi: 10.1093/nar/2.5.699.

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Polynucleotide analogs: acrylic acid and maleic acid copolymers of 1-vinyluracil and 9-vinyladenine.

Biochemistry. 1975 Nov 4;14(22):4892-6. doi: 10.1021/bi00693a017.

8

Template specific inhibitor of mammalian DNA polymerases.哺乳动物DNA聚合酶的模板特异性抑制剂。

Nucleic Acids Res. 1976 Mar;3(3):825-34. doi: 10.1093/nar/3.3.825.

9

Mechanims of stimulation of in vitro protein synthesis by some copolymers of styrene, vinyluracil, and vinyladenine with maleic acid and acrylic acid.某些苯乙烯、乙烯基尿嘧啶和乙烯基腺嘌呤与马来酸和丙烯酸的共聚物刺激体外蛋白质合成的机制。

Biochemistry. 1976 Aug 10;15(16):3536-40. doi: 10.1021/bi00661a022.

10

Effects of Poly(1-vinyluracil) and Poly(9-vinyladenine) on viral RNA-directed DNA polymerase.聚（1-乙烯基尿嘧啶）和聚（9-乙烯基腺嘌呤）对病毒RNA指导的DNA聚合酶的影响。

Cancer Res. 1976 Apr;36(4):1273-7.

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