体外淋巴细胞转化。I. 组织培养条件及定量测定
Lymphocyte transformation in vitro. I. Tissue culture conditions and quantitative measurements.
作者信息
Schellekens P T, Eijsvoogel V P
出版信息
Clin Exp Immunol. 1968 Jul;3(6):571-84.
Abstract
The quantitative evaluation of the reactivity in human PHA-stimulated lymphocyte cultures was studied. A technique for the measurement of DNA synthesis by means of thymidine-2-C incorporation was standardized and its reproducibility determined. Using this method a number of variables in culture conditions were investigated. The results obtained are discussed.
摘要
对人PHA刺激淋巴细胞培养物中的反应性进行了定量评估。通过胸苷-2-C掺入来测量DNA合成的技术得到了标准化，并确定了其可重复性。使用该方法研究了培养条件中的一些变量。对所获得的结果进行了讨论。
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