体外淋巴细胞转化定量评估的显微技术
Microtechnique for quantitative evaluation of in vitro lymphocyte transformation.
作者信息
Junge U, Hoekstra J, Wolfe L, Deinhardt F
出版信息
Clin Exp Immunol. 1970 Sep;7(3):431-7.
Abstract
A microtechnique is described to quantitate transformation of lymphocytes from man and marmosets following stimulation by PHA and different antigens. The C-thymidine uptake of lymphocytes grown in cultures of 0·06–0·2 ml of whole blood was measured by liquid scintillation. The magnitude, consistency and reproducibility of the results were similar to those achieved using cultures of purified lymphocytes or cell-rich plasma but fewer technical procedures were required.
摘要
本文描述了一种微量技术，用于定量人及狨猴淋巴细胞在PHA和不同抗原刺激后的转化情况。通过液体闪烁法测量在0·06–0·2 ml全血培养物中生长的淋巴细胞的C-胸苷摄取量。结果的大小、一致性和可重复性与使用纯化淋巴细胞或富含细胞的血浆培养物所获得的结果相似，但所需的技术步骤更少。
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本文引用的文献
1
2
3
[A simplified technic of lymphocyte culture as an allergy test. The micro-whole blood technic].
Hautarzt. 1967 Oct;18(10):459-61.
4
Immunization against mumps.
Prog Med Virol. 1969;11:126-53.
5
Culture of normal human leukocytes.正常人白细胞培养。
JAMA. 1967 Feb 20;199(8):519-24.
6
Death and division of lymphocytes. Neglected factors in assessment of P.H.A.-induced transformation.
Lancet. 1968 Nov 23;2(7578):1120-3. doi: 10.1016/s0140-6736(68)91583-3.
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