Junge U, Hoekstra J, Wolfe L, Deinhardt F
Clin Exp Immunol. 1970 Sep;7(3):431-7.
A microtechnique is described to quantitate transformation of lymphocytes from man and marmosets following stimulation by PHA and different antigens. The C-thymidine uptake of lymphocytes grown in cultures of 0·06–0·2 ml of whole blood was measured by liquid scintillation. The magnitude, consistency and reproducibility of the results were similar to those achieved using cultures of purified lymphocytes or cell-rich plasma but fewer technical procedures were required.
本文描述了一种微量技术，用于定量人及狨猴淋巴细胞在PHA和不同抗原刺激后的转化情况。通过液体闪烁法测量在0·06–0·2 ml全血培养物中生长的淋巴细胞的C-胸苷摄取量。结果的大小、一致性和可重复性与使用纯化淋巴细胞或富含细胞的血浆培养物所获得的结果相似，但所需的技术步骤更少。
