Befus A D, O'Neill M, Bienenstock J
Immunology. 1978 Dec;35(6):901-6.
Immunofluorscence studies have shown that injection of lymphocytes from either the intestinal lamina propria (LP) or from the Peyer's patches (PP) into irradiated (1000-1250 rad) allogeneic rabbits leads to the differentiation and proliferation of IgA containing cells in the spleen, mesenteric lymph node (MLN) and intestine by day 6 and to a lesser extent by day 4. In contrast, few IgA containing cells were seen in irradiated animals not given lymphocytes or given popliteal lymph node cells. Transfer of PP lymphocytes resulted in statistically greater numbers of IgA containing cells in the recipient MLN or spleen than did transfer of LP lymphocytes. In the PP of recipient rabbits given either LP or PP lymphocytes, intercellular IgA was abundant and more IgA containing cells were seen near the PP than in sites distant from PP. These results show that IgA precursor cells are present in the intestinal LP as well as in the PP. The nature and distribution of the IgA precursor and the characteristics of the IgA repopulation are discussed.
免疫荧光研究表明，将来自肠固有层（LP）或派尔集合淋巴结（PP）的淋巴细胞注射到经辐射（1000 - 1250拉德）的同种异体兔体内，到第6天时，脾脏、肠系膜淋巴结（MLN）和肠道中含IgA的细胞会发生分化和增殖，到第4天时程度较轻。相比之下，在未给予淋巴细胞或给予腘窝淋巴结细胞的辐射动物中，很少能见到含IgA的细胞。与LP淋巴细胞转移相比，PP淋巴细胞转移导致受体MLN或脾脏中含IgA的细胞数量在统计学上更多。在接受LP或PP淋巴细胞的受体兔的PP中，细胞间IgA丰富，且在PP附近比远离PP的部位可见到更多含IgA的细胞。这些结果表明，IgA前体细胞存在于肠LP以及PP中。本文讨论了IgA前体的性质和分布以及IgA再填充的特征。
