胞壁质脂蛋白的抗原决定簇及其在肠杆菌科细菌表面的暴露情况。

Antigenic determinants of murein lipoprotein and its exposure at the surface of Enterobacteriaceae.

作者信息

Braun V, Bosch V, Klumpp E R, Neff I, Mayer H, Schlecht S

出版信息

Eur J Biochem. 1976 Mar 1;62(3):555-66. doi: 10.1111/j.1432-1033.1976.tb10190.x.

DOI:10.1111/j.1432-1033.1976.tb10190.x

Abstract

Murein lipoprotein from the outer membrane of Escherichia coli could be fixed to erythrocytes without pretreatment of the erythrocytes. Passive hemagglutination or immune hemolysis could thus be used as sensitive assays to determine antibodies against lipoprotein. In rabbit antisera prepared against whole cells of E. coli, Salmonella, Arizona, and Shigella antibodies against lipoprotein were present. The respective titers were lowest in encapsulated smooth strains and highest in rough mutants. Antisera against deep rough mutants showed even higher anti-lipoprotein titers than anti-R-lipopolysaccharide titers. Correspondingly,absorption of lipoprotein antibodies with enterobacterial strains was most pronounced with deep rough mutants and lowest with smooth strains. Lipoprotein becomes increasingly an immunogen as well as an antigen the more sugar residues are missing in the lipolysaccharide on the cell surface. In wild-type cells lipoprotein is buried in the outer membrane; its exposure in mutant cells is related to defects at the cell surface.

摘要

来自大肠杆菌外膜的胞壁质脂蛋白无需对红细胞进行预处理就能固定在红细胞上。因此，被动血凝或免疫溶血可作为灵敏的检测方法来测定抗脂蛋白抗体。在针对大肠杆菌、沙门氏菌、亚利桑那菌和志贺氏菌全细胞制备的兔抗血清中，存在抗脂蛋白抗体。在有荚膜的光滑菌株中，各自的效价最低，而在粗糙突变体中最高。针对深度粗糙突变体的抗血清显示出比对R-脂多糖的效价更高的抗脂蛋白效价。相应地，用肠道细菌菌株吸收脂蛋白抗体时，深度粗糙突变体最为明显，而光滑菌株最低。细胞表面脂多糖中缺失的糖残基越多，脂蛋白就越成为一种免疫原以及抗原。在野生型细胞中，脂蛋白埋藏在外膜中；其在突变体细胞中的暴露与细胞表面的缺陷有关。