Pack B A, Christensen C, Douraghy M, Brooks S C
Endocrinology. 1978 Dec;103(6):2129-36. doi: 10.1210/endo-103-6-2129.
The levels of nuclear and uncharged cytoplasmic estrogen receptors were determined in gilt endometrium throughout the estrous cycle. Nuclear estrogen receptor was found to reach its highest level of 1.3 (range, 1.0-1.5) pmol/mg DNA on day 1 of the cycle just before ovulation (day 2), after which the values dropped sharply to 0.3 (range, 0.1-0.4) pmol/mg DNA. This level of nuclear receptor was consistently found through day 9. Between days 9-14, the estrogen receptor present in the nucleus decreased to zero, where it remained until the return of estrus (day 1). The dissociation constant (Kd +/- SD) for the nuclear estrogen receptor was 1.6 (+/- 1.4) x 10(-9) M. Initially, the nuclear estrogen receptor peak at day 1 was preceded by elevation (0.04-0.05 pmol/mg DNA) in cytoplasmic receptor at day 19, which declined when the receptor appeared in the nucleus. There was an even larger peak (range of maximal values, 0.07-0.26 pmol/mg DNA) of uncharged receptor in the cytosol near day 5. Throughout the rest of the cycle the values ranged from 0.01-0.03 pmol/mg DNA. Kd( +/- SD) for the cytoplasmic estrogen receptor was 2.4 (+/- 1.5) x 10(-9) M. The fluctuations of these two pools of endometrial estrogen receptor are discussed in relation to the previously observed cyclic variations in porcine uterine oxidation and sulfurylation of 17 beta-estradiol (Pack and Brooks, Endocrinology 95: 1680, 1974).
在整个发情周期中，测定了后备母猪子宫内膜中核雌激素受体和非结合型胞质雌激素受体的水平。发现核雌激素受体在发情周期第1天（排卵前一天，即第2天）达到最高水平，为1.3（范围1.0 - 1.5）pmol/mg DNA，之后该值急剧下降至0.3（范围0.1 - 0.4）pmol/mg DNA。直至第9天，核受体水平一直维持在此水平。在第9 - 14天之间，核内的雌激素受体降至零，并一直保持到发情期（第1天）再次出现。核雌激素受体的解离常数（Kd +/- SD）为1.6（+/- 1.4）x 10(-9) M。最初，第1天核雌激素受体达到峰值之前，第19天胞质受体出现升高（0.04 - 0.05 pmol/mg DNA），当受体出现在细胞核时，该值下降。在第5天左右，胞质中未结合受体出现更大的峰值（最大值范围为0.07 - 0.26 pmol/mg DNA）。在发情周期的其余时间里，该值范围为0.01 - 0.03 pmol/mg DNA。胞质雌激素受体的Kd（+/- SD）为2.4（+/- 1.5）x 10(-9) M。结合先前观察到的猪子宫中17β - 雌二醇氧化和硫酸化的周期性变化（Pack和Brooks，《内分泌学》95: 1680，1974），讨论了子宫内膜雌激素受体这两个库的波动情况。
