聚2'-O-乙基胞苷酸的制备与性质
Preparation and properties of poly 2'-O-ethylcytidylic acid.
作者信息
Kielanowska M, Shugar D
出版信息
Nucleic Acids Res. 1976 Mar;3(3):817-24. doi: 10.1093/nar/3.3.817.
Abstract
Poly 2'0-ethylcytidylic acid (poly (Ce)) was prepared by polymerization of 2'-0-ethylcytidine-5'-pyrophosphate with Escherichia coli polynucleotide phosphorylase in the presence of Mn++, and its properties compared with those of poly (rC), poly (Cm) and poly (dC). The neutral form of pOLY (Ce) exhibits properties similar to those of poly (rC) and poly (Cm). It also forms an acid twin-stranded helix with a transition pH of 5.9 in 0.1 M NaCl. The neutral form readily forms a double-stranded helical complex with poly (rI). Relative to poly (Cm), replacement of the 2'-0-methyl by 2-0-ethyl leads to increased enhancement of the thermal stabilities of both the acid helical form of poly (Ce) and its complex with poly (rI).
摘要
聚2'-O-乙基胞苷酸（聚（Ce））是通过在Mn++存在下，用大肠杆菌多核苷酸磷酸化酶使2'-O-乙基胞苷-5'-焦磷酸聚合制备的，并将其性质与聚（rC）、聚（Cm）和聚（dC）的性质进行了比较。聚（Ce）的中性形式表现出与聚（rC）和聚（Cm）相似的性质。它还能形成一种酸性双链螺旋，在0.1M NaCl中的转变pH为5.9。中性形式很容易与聚（rI）形成双链螺旋复合物。相对于聚（Cm），用2-O-乙基取代2'-O-甲基会导致聚（Ce）的酸性螺旋形式及其与聚（rI）的复合物的热稳定性增强。
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本文引用的文献
1
THE TAUTOMERIC FORM OF HELICAL POLYRIBOCYTIDYLIC ACID.螺旋状聚核糖胞苷酸的互变异构形式。
J Am Chem Soc. 1965 May 5;87:2033-9. doi: 10.1021/ja01087a031.
2
Properties of helical polycytidylic acid.螺旋状聚胞苷酸的特性。
Biochemistry. 1963 Mar-Apr;2:340-4. doi: 10.1021/bi00902a028.
3
Spectrophotometric studies of nucleic acid derivatives and related compounds as a function of pH. II. Natural and synthetic pyrimidine nucleosides.核酸衍生物及相关化合物作为pH函数的分光光度研究。II. 天然和合成嘧啶核苷。
Biochim Biophys Acta. 1952 Oct;9(4):369-84. doi: 10.1016/0006-3002(52)90181-9.
4
The structure of poly-5-methylcytidylic acid and its twin-stranded complex with poly-inosinic acid.聚5-甲基胞苷酸的结构及其与聚肌苷酸的双链复合物。
J Mol Biol. 1966 May;17(1):174-87. doi: 10.1016/s0022-2836(66)80102-x.
5
Methylated bases and sugars in 16-S and 28-S RNA from L cells.来自L细胞的16-S和28-S RNA中的甲基化碱基和糖。
Biochim Biophys Acta. 1969 Apr 22;179(2):332-40. doi: 10.1016/0005-2787(69)90041-0.
6
Studies on the incorporation of L-ethionine-ethyl-l-14C into the transfer RNA of rat liver.关于将L-乙硫氨酸-乙基-L-14C掺入大鼠肝脏转运RNA的研究。
Cancer Res. 1969 Feb;29(2):380-90.
7
Poly 2'-O-methylcytidylic acid and the role of the 2'-hydroxyl in polynucleotide structure.聚2'-O-甲基胞苷酸以及2'-羟基在多核苷酸结构中的作用。
Biochem Biophys Res Commun. 1969 Dec 4;37(6):895-901. doi: 10.1016/0006-291x(69)90215-0.
8
Physical and chemical characterization of two- and three-stranded adenine-thymine and adenine-uracil homopolymer complexes.双链和三链腺嘌呤-胸腺嘧啶以及腺嘌呤-尿嘧啶同聚物复合物的物理和化学特性
J Mol Biol. 1966 Sep;20(2):359-89. doi: 10.1016/0022-2836(66)90069-6.
9
Role of the 2'-hydroxyl in polynucleotide conformation: poly 2'-O-methyluridylic acid.2'-羟基在多核苷酸构象中的作用：聚2'-O-甲基尿苷酸
Acta Biochim Pol. 1971;18(3):321-37.
10
Influence of 2'-O-alkylation on the structure of single-stranded polynucleotides and the stability of 2'-O-alkylated polynucleotide complexes.2'-O-烷基化对单链多核苷酸结构及2'-O-烷基化多核苷酸复合物稳定性的影响
Biochemistry. 1974 Jun 18;13(13):2762-71. doi: 10.1021/bi00710a016.
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