Kielanowska M, Shugar D
Nucleic Acids Res. 1976 Mar;3(3):817-24. doi: 10.1093/nar/3.3.817.
Poly 2'0-ethylcytidylic acid (poly (Ce)) was prepared by polymerization of 2'-0-ethylcytidine-5'-pyrophosphate with Escherichia coli polynucleotide phosphorylase in the presence of Mn++, and its properties compared with those of poly (rC), poly (Cm) and poly (dC). The neutral form of pOLY (Ce) exhibits properties similar to those of poly (rC) and poly (Cm). It also forms an acid twin-stranded helix with a transition pH of 5.9 in 0.1 M NaCl. The neutral form readily forms a double-stranded helical complex with poly (rI). Relative to poly (Cm), replacement of the 2'-0-methyl by 2-0-ethyl leads to increased enhancement of the thermal stabilities of both the acid helical form of poly (Ce) and its complex with poly (rI).
聚2'-O-乙基胞苷酸(聚(Ce))是通过在Mn++存在下,用大肠杆菌多核苷酸磷酸化酶使2'-O-乙基胞苷-5'-焦磷酸聚合制备的,并将其性质与聚(rC)、聚(Cm)和聚(dC)的性质进行了比较。聚(Ce)的中性形式表现出与聚(rC)和聚(Cm)相似的性质。它还能形成一种酸性双链螺旋,在0.1M NaCl中的转变pH为5.9。中性形式很容易与聚(rI)形成双链螺旋复合物。相对于聚(Cm),用2-O-乙基取代2'-O-甲基会导致聚(Ce)的酸性螺旋形式及其与聚(rI)的复合物的热稳定性增强。