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多核苷酸。LVII。聚（2'-氯-2'-脱氧肌苷酸）的合成与性质

Polynucleotides. LVII. Synthesis and properties of poly (2'-chloro-2'-deoxyinosinic acid).

作者信息

Kakiuchi N, Fukui T, Ikehara M

出版信息

Nucleic Acids Res. 1979 Jun 11;6(7):2627-36. doi: 10.1093/nar/6.7.2627.

DOI:10.1093/nar/6.7.2627
PMID:461198
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327876/
Abstract

Poly (2'-chloro-2'-deoxyinosinic acid) [poly(Icl)] was synthesized from Icl 5'-DP by polymerization with polynucleotide phosphorylase. UV absorption properties of poly(Icl) are very similar to those of poly(I). Poly(Icl) adopted a multi-stranded ordered form in the presence of 0.95M Na ion. The Tm value of this form was 36 degrees, which resembles that of poly(I) quadruple-stranded form at high salt. CD spectra also suggested presence of these two forms. Upon mixing with poly(C), poly-(Icl) forms a double-stranded 1 : 1 complex, which had very similar Tm-log[Na+] relationship to that of poly(I) . poly(C). Thus it was concluded that the chlorine substitution at 2'-position of the polynucleotide had the similar effect to OH on physical properties of polynucleotides.

摘要

聚（2'-氯-2'-脱氧肌苷酸）[聚（Icl）]由Icl 5'-二磷酸通过与多核苷酸磷酸化酶聚合反应合成。聚（Icl）的紫外吸收特性与聚（I）非常相似。在0.95M钠离子存在下，聚（Icl）呈现多链有序形式。这种形式的熔解温度（Tm）值为36摄氏度，这与聚（I）在高盐条件下的四重链形式相似。圆二色光谱（CD光谱）也表明存在这两种形式。与聚（C）混合时，聚（Icl）形成双链1:1复合物，其Tm-log[Na⁺]关系与聚（I）·聚（C）非常相似。因此得出结论，多核苷酸2'-位的氯取代对多核苷酸物理性质的影响与羟基相似。

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本文引用的文献

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PHYSICAL AND CHEMICAL CHARACTERIZATION OF THE ORDERED COMPLEXES FORMED BETWEEN POLYINOSINIC ACID, POLYCYTIDYLIC ACID AND THEIR DEOXYRIBO-ANALOGUES.多聚肌苷酸、多聚胞苷酸及其脱氧核糖类似物之间形成的有序复合物的物理和化学特性
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The molecular structure of polyinosinic acid.聚肌苷酸的分子结构。
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Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels.在琼脂糖-丙烯酰胺复合凝胶中通过电泳进行核糖核酸的分子量估计及分离
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The structures of polyinosinic acid.聚肌苷酸的结构。
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Polynucleotides containing 2'-chloro-2'-deoxyribose.含有2'-氯-2'-脱氧核糖的多核苷酸。
Biochemistry. 1972 Nov 7;11(23):4336-44. doi: 10.1021/bi00773a021.
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The antiviral activity of certain thiophosphate and 2'-chloro substituted polynucleotide homopolymer duplexes.某些硫代磷酸酯和2'-氯取代的多核苷酸均聚物双链体的抗病毒活性。
Virology. 1972 May;48(2):537-45. doi: 10.1016/0042-6822(72)90064-5.
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Polynucleotides. XLV Synthesis and properties of poly(2'-azido-2'-deoxyinosinic acid).多核苷酸。XLV. 聚（2'-叠氮基-2'-脱氧肌苷酸）的合成与性质
Nucleic Acids Res. 1977 Aug;4(8):2629-39. doi: 10.1093/nar/4.8.2629.
9
Polynucleotides. LVI. Synthesis and properties of poly(2-deoxy-2'-fluoroinosinic acid).多核苷酸。LVI。聚（2-脱氧-2'-氟肌苷酸）的合成与性质
Nucleic Acids Res. 1978 Sep;5(9):3315-24. doi: 10.1093/nar/5.9.3315.
10
Polynucleotides. LII. Synthesis and properties of poly(2'-deoxy-2'-fluoroadenylic acid).多核苷酸。LII。聚（2'-脱氧-2'-氟腺苷酸）的合成与性质
Nucleic Acids Res. 1978 Jun;5(6):1877-87. doi: 10.1093/nar/5.6.1877.

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