Kakiuchi N, Fukui T, Ikehara M
Nucleic Acids Res. 1979 Jun 11;6(7):2627-36. doi: 10.1093/nar/6.7.2627.
Poly (2'-chloro-2'-deoxyinosinic acid) [poly(Icl)] was synthesized from Icl 5'-DP by polymerization with polynucleotide phosphorylase. UV absorption properties of poly(Icl) are very similar to those of poly(I). Poly(Icl) adopted a multi-stranded ordered form in the presence of 0.95M Na ion. The Tm value of this form was 36 degrees, which resembles that of poly(I) quadruple-stranded form at high salt. CD spectra also suggested presence of these two forms. Upon mixing with poly(C), poly-(Icl) forms a double-stranded 1 : 1 complex, which had very similar Tm-log[Na+] relationship to that of poly(I) . poly(C). Thus it was concluded that the chlorine substitution at 2'-position of the polynucleotide had the similar effect to OH on physical properties of polynucleotides.
聚(2'-氯-2'-脱氧肌苷酸)[聚(Icl)]由Icl 5'-二磷酸通过与多核苷酸磷酸化酶聚合反应合成。聚(Icl)的紫外吸收特性与聚(I)非常相似。在0.95M钠离子存在下,聚(Icl)呈现多链有序形式。这种形式的熔解温度(Tm)值为36摄氏度,这与聚(I)在高盐条件下的四重链形式相似。圆二色光谱(CD光谱)也表明存在这两种形式。与聚(C)混合时,聚(Icl)形成双链1:1复合物,其Tm-log[Na⁺]关系与聚(I)·聚(C)非常相似。因此得出结论,多核苷酸2'-位的氯取代对多核苷酸物理性质的影响与羟基相似。