多核苷酸。XLIV。聚（2-氮杂腺苷酸）和聚（2-氮杂肌苷酸）的合成及性质

Polynucleotides. XLIV. Synthesis and properties of poly (2-azaadenylic acid) and poly(2-azainosinic acid).

作者信息

Fukui T, Kakiuchi N, Ikehara M

出版信息

Biochim Biophys Acta. 1978 Sep 27;520(2):441-51. doi: 10.1016/0005-2787(78)90241-1.

Abstract

Chemically synthesized 2-azaadenosine 5'-diphosphate (n2ADP) and 2-azainosine 5'-diphosphate (n2IDP) were polymerized to yield poly(2-azaadenylic acid), poly(n2A), and poly(2-azainosinic acid), poly(n2I), using Escherichia coli polynucleotide phosphorylase. In neutral solution, poly(n2A) and poly(n2I) had hypochromicities of 32 and 5.5%, respectively. Poly(n2A) formed an ordered structure, which had a melting temperature (Rm) of 20 degrees C at 0.15 M salt concentration. Upon mixing with poly(U), poly(n2A) formed a 1 : 2 complex with Tm of 41 degrees C at 0.15 M salt concentration. Poly(n2A) and poly(n2I) formed three-stranded complexes with poly(I), and poly(A), respectively. Poly(n2A) . 2poly(I), poly(A) . 2poly(n2I), and poly(n2A) . 2poly(n2I) complexes had Tm values of 23, 48, and 31 degrees C at 0.15 M salt concentration, respectively. Poly(n2I) formed a double-stranded complex with poly(C), but its Tm was very low.

摘要

利用大肠杆菌多核苷酸磷酸化酶将化学合成的2-氮杂腺苷5'-二磷酸（n2ADP）和2-氮杂肌苷5'-二磷酸（n2IDP）聚合，分别得到聚（2-氮杂腺苷酸），聚（n2A）和聚（2-氮杂肌苷酸），聚（n2I）。在中性溶液中，聚（n2A）和聚（n2I）的减色率分别为32%和5.5%。聚（n2A）形成有序结构，在0.15M盐浓度下其解链温度（Rm）为20℃。与聚（U）混合时，聚（n2A）在0.15M盐浓度下形成1:2复合物，其熔解温度（Tm）为41℃。聚（n2A）和聚（n2I）分别与聚（I）和聚（A）形成三链复合物。聚（n2A）·2聚（I）、聚（A）·2聚（n2I）和聚（n2A）·2聚（n2I）复合物在0.15M盐浓度下的Tm值分别为23℃、48℃和31℃。聚（n2I）与聚（C）形成双链复合物，但其Tm值非常低。