从人体中获取完整的雄性和雌性链尾双瓣线虫。
Recovery of intact male and female Dipetalonema streptocerca from man.
作者信息
Gardiner C H, Meyers W M, Lanoie L O
出版信息
Am J Trop Med Hyg. 1979 Jan;28(1):49-52. doi: 10.4269/ajtmh.1979.28.49.
When attempts at teasing adult Dipetalonema streptocerca free from biopsy specimens of human skin proved futile a digestion procedure was initiated. Punch biopsy specimens fixed in Michel's solution (ammonium sulfate) were incubated at 25 degrees C for 3 days in a 1.0% solution of collagenase in tris-HCl buffer. Intact worms were carefully teased out of the digested collagen and camera lucida drawings and measurements were then possible. This marks the first description of intact D. streptocerca adults recovered from man.
当尝试从人类皮肤活检标本中挑出成年链尾双瓣线虫却徒劳无功时，便启动了消化程序。将固定在米歇尔溶液（硫酸铵）中的打孔活检标本在25摄氏度下于含有1.0%胶原酶的三羟甲基氨基甲烷盐酸盐缓冲液中孵育3天。完整的虫体被小心地从消化后的胶原中挑出，然后便可以进行摄像绘图和测量。这标志着首次描述了从人体中 recovered 的完整链尾双瓣线虫成虫。 （注：“recovered”在这里结合语境推测可能是“获取、得到”之类的意思，但原英文表述不太完整准确，按要求完整翻译了给定英文内容）
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