Beltramino C, Taleisnik S
Neuroendocrinology. 1979;28(5):320-8. doi: 10.1159/000122879.
The olfactory bulbs were electrochemically stimulated in non-anesthetized, freely moving rats, and the ovulatory response and serum luteinizing hormone (LH) levels were studied. The stimulus was applied at 11:30 h through unilateral stainless steel electrodes chronically implanted, and serial blood samples were obtained by way of a plastic cannula located in the jugular vein. Electrochemical stimulation (100 microA/60 sec) of the main olfactory bulbs in the ventral aspect of the superficial layers as well as in its central core, performed on the day of proestrus, prevented the preovulatory discharge of LH and ovulation. Blockade of ovulation also occurred when the stimulus was applied in the lateral or medial parts of the superficial layers. On the other hand stimulation in the dorsal part blocked the preovulatory surge of LH and ovulation in only 50% of the cases. Stimulation applied in the accessory olfactory bulbs failed to interfere with ovulation and LH discharge. Stimulation of ovariectomized estrogen-primed rats applied in superficial layers of the main olfactory bulbs had no significant effect on the release of LH, but when the stimulus was applied in the accessory olfactory bulb a release of LH took place 3-6 h following stimulation. These results indicate that stimulation of the olfactory bulbs produce a dual effect on LH release: while the response of the accessory olfactory bulb is facilitatory in nature, that of the main olfactory bulbs is inhibitory.
在未麻醉、自由活动的大鼠中对嗅球进行电化学刺激，并研究排卵反应和血清促黄体生成素（LH）水平。刺激于上午11:30通过长期植入的单侧不锈钢电极施加，通过位于颈静脉的塑料插管获取系列血样。在动情前期当天，对表层腹侧以及中央核心部位的主嗅球进行电化学刺激（100微安/60秒），可阻止LH的排卵前释放及排卵。当在表层的外侧或内侧部位施加刺激时，也会出现排卵受阻。另一方面，在背侧部位进行刺激时，仅50%的情况下会阻断LH的排卵前激增及排卵。对副嗅球施加刺激未能干扰排卵和LH释放。对去卵巢并用雌激素预处理的大鼠，在主嗅球表层施加刺激对LH释放无显著影响，但当在副嗅球施加刺激时，刺激后3至6小时会出现LH释放。这些结果表明，对嗅球的刺激对LH释放产生双重作用：副嗅球的反应本质上是促进性的，而主嗅球的反应是抑制性的。
