Carter C E, Colley D G
J Immunol. 1979 Jun;122(6):2204-9.
A crude preparation of Schistosoma mansoni soluble egg antigen (SEA) was subjected to affinity chromatography with concanavalin A (Con A) bound to Sepharose 4B. The resulting Con A fractions (bound and unbound) were characterized with sodium dodecyl sulfate (SDS) gel electrophoresis, immunoelectrophoresis, immunodiffusion, and lymphocyte blastogenesis techniques. In the fraction that did not bind to Con A there were at least two distinct antigens, and there were also at least two distinct antigens in the fractions that did bind to Con A. With SDS polyacrylamide gel electrophoresis, at least 20 distinct protein bands (Coomassie blue staining) and three glycoprotein bands (PAS reactive) were present in the unbound fractions from Con A chromatography. The bound fractions separated into at least six distinct glycoproteins with SDS electrophoresis. Although both the bound and unbound fractions contained precipitating antigens, only the bound fractions were capable of eliciting lymphocyte blastogenic responses.
用结合在琼脂糖4B上的伴刀豆球蛋白A（Con A）对曼氏血吸虫可溶性虫卵抗原（SEA）的粗制品进行亲和层析。用十二烷基硫酸钠（SDS）凝胶电泳、免疫电泳、免疫扩散和淋巴细胞增殖技术对所得的Con A组分（结合和未结合的）进行表征。在未与Con A结合的组分中至少有两种不同的抗原，在与Con A结合的组分中也至少有两种不同的抗原。通过SDS聚丙烯酰胺凝胶电泳，Con A层析未结合组分中存在至少20条不同的蛋白条带（考马斯亮蓝染色）和三条糖蛋白条带（PAS反应性）。通过SDS电泳，结合组分分离成至少六种不同的糖蛋白。虽然结合和未结合组分都含有沉淀抗原，但只有结合组分能够引发淋巴细胞增殖反应。
