Persson L I, Rosengren L E, Johansson B B, Hansson H A
J Neurol Sci. 1979 Jun;42(1):65-72. doi: 10.1016/0022-510x(79)90152-7.
Ethanol-intoxicated and non-intoxicated rats were injected with horseradish peroxidase and Evan's blue-labelled albumin and given a small air embolus in the right common carotid artery after ligation of the external carotid branch. In both ethanol-intoxicated and non-intoxicated rats, some endothelial cells, mainly in arteries and arterioles, showed a diffuse distribution of peroxidase in the cytoplasm. In some arterioles with a diffuse endothelial distribution of peroxidase there was a detachment of endothelial cells from the vessel wall, with an exposure of the adluminal basement membrane to blood elements. This endothelial detachment was mainly observed in ethanol-intoxicated rats. The vascular basement membranes underlying detached endothelial cells contained peroxidase, both in ethanol-intoxicated and in non-intoxicated rats. There was a considerable leakage of peroxidase via endothelial pinocytotic vesicles into the vascular basement membranes, mainly in arterioles, but also in capillaries and venules of the embolised hemisphere. This transendothelial pinocytotic transport of peroxidase was more prominent in ethanol-intoxicated than in non-intoxicated rats.
给乙醇中毒和未中毒的大鼠注射辣根过氧化物酶和伊文思蓝标记的白蛋白，并在结扎颈外分支后，经右侧颈总动脉给予小空气栓子。在乙醇中毒和未中毒的大鼠中，一些内皮细胞（主要在动脉和小动脉中）的细胞质中显示出过氧化物酶的弥漫性分布。在一些过氧化物酶在内皮细胞中呈弥漫性分布的小动脉中，内皮细胞从血管壁脱离，使管腔侧基底膜暴露于血液成分。这种内皮细胞脱离主要在乙醇中毒的大鼠中观察到。在乙醇中毒和未中毒的大鼠中，脱离的内皮细胞下方的血管基底膜均含有过氧化物酶。过氧化物酶通过内皮细胞的胞饮小泡大量渗漏到血管基底膜中，主要发生在小动脉中，但也见于栓塞半球的毛细血管和小静脉。与未中毒的大鼠相比，乙醇中毒的大鼠中这种过氧化物酶的跨内皮细胞胞饮转运更为明显。
