关于氨基酸的15N核磁共振研究。
15N nuclear magnetic resonance investigations on amino acids.
作者信息
Blomberg F, Maurer W, Rüterjans H
出版信息
Proc Natl Acad Sci U S A. 1976 May;73(5):1409-13. doi: 10.1073/pnas.73.5.1409.
Abstract
15N nuclear magnetic resonance investigations of some amino acids were carried out in order to check the applicability of this method to biological problems. Because the natural abundance of the 15N isotope is not sufficient to get readable spectra in a reasonable time, 95% 15N isotope-enriched samples were used for the measurements. Besides the chemical shift values, the line widths, and the nuclear Overhauser enhancement factors, spin lattice relaxation times of the correspondent 15N resonances were measured as functions of pH had temperature.
摘要
为了检验这种方法对生物学问题的适用性，对一些氨基酸进行了15N核磁共振研究。由于15N同位素的天然丰度不足以在合理时间内获得可读光谱，因此使用95% 15N同位素富集的样品进行测量。除了化学位移值、线宽和核Overhauser增强因子外，还测量了相应15N共振的自旋晶格弛豫时间作为pH和温度的函数。
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