犬下颌下腺透明质酸酶。纯化及特性
Canine submandibular-gland hyaluronidase. Purification and properties.
作者信息
Tan Y H, Bowness J M
出版信息
Biochem J. 1968 Nov;110(1):19-25. doi: 10.1042/bj1100019.
Abstract
- Methods for the purification of dog submandibular-gland hyaluronidase from sedimentable and non-sedimentable portions of a homogenate and from the whole homogenate are presented. The method consists of three main steps: removal of mucin by acid precipitation or gel filtration on Sephadex G-200, ammonium sulphate precipitation and CM-cellulose chromatography. By this method specific activities of up to 1.28 and 0.78mumoles of N-acetylglucosamine/min./mg. of protein were obtained for the purified freeze-dried non-sedimentable hyaluronidase and for the sedimentable hyaluronidase respectively. 2. A comparison of some of the properties of the non-sedimentable and the sedimentable hyaluronidase preparation indicated that there was little difference between the two and that they both resembled lysosomal hyaluronidase from rat liver.
摘要
- 本文介绍了从匀浆的可沉淀部分和不可沉淀部分以及整个匀浆中纯化狗下颌下腺透明质酸酶的方法。该方法包括三个主要步骤：通过酸沉淀或在葡聚糖G - 200上进行凝胶过滤去除粘蛋白、硫酸铵沉淀和CM - 纤维素色谱法。通过这种方法，纯化的冻干不可沉淀透明质酸酶和可沉淀透明质酸酶的比活性分别达到1.28和0.78微摩尔N - 乙酰葡糖胺/分钟/毫克蛋白质。2. 对不可沉淀和可沉淀透明质酸酶制剂的一些性质进行比较表明，两者之间差异不大，并且它们都类似于大鼠肝脏的溶酶体透明质酸酶。
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本文引用的文献
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