骨组织溶酶体中的透明质酸酶活性。
Hyaluronidase activity in lysosomes of bone tissue.
作者信息
Vaes G
出版信息
Biochem J. 1967 Jun;103(3):802-4. doi: 10.1042/bj1030802.
Abstract
- The distribution pattern of hyaluronidase in subcellular fractions of bone-tissue homogenates is closely similar to that reported by Vaes & Jacques (1965b) for the other acid hydrolases of this tissue. The highest specific activity of hyaluronidase is also found in the light-mitochondrial fraction. 2. In cytoplasmic extracts of bone, about 60% of the activity of hyaluronidase is latent, and is unmasked by a number of treatments (digitonin, low osmotic pressure, freezing and thawing, Waring Blendor) that unmask the lysosomal beta-glucuronidase in a closely parallel manner. Low concentrations of Triton X-100 render a larger proportion of beta-glucuronidase than of hyaluronidase accessible to external substrates, but release the same proportion of both enzymes in unsedimentable form. 3. These results support the concept of an association of hyaluronidase with lysosomes in bone.
摘要
- 透明质酸酶在骨组织匀浆亚细胞组分中的分布模式与Vaes和Jacques（1965b）报道的该组织其他酸性水解酶的分布模式极为相似。透明质酸酶的最高比活性也出现在轻线粒体组分中。2. 在骨的细胞质提取物中，约60%的透明质酸酶活性是潜伏性的，并且通过一些处理（洋地黄皂苷、低渗透压、冻融、韦林搅拌器）可将其暴露出来，这些处理以非常相似的方式使溶酶体β-葡萄糖醛酸酶暴露。低浓度的曲拉通X-100使更多比例的β-葡萄糖醛酸酶而非透明质酸酶能够接触到外部底物，但以不可沉降形式释放的两种酶的比例相同。3. 这些结果支持骨中透明质酸酶与溶酶体相关联的概念。
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