Wojtas David H, Ayyer Kartik, Liang Mengning, Mossou Estelle, Romoli Filippo, Seuring Carolin, Beyerlein Kenneth R, Bean Richard J, Morgan Andrew J, Oberthuer Dominik, Fleckenstein Holger, Heymann Michael, Gati Cornelius, Yefanov Oleksandr, Barthelmess Miriam, Ornithopoulou Eirini, Galli Lorenzo, Xavier P Lourdu, Ling Wai Li, Frank Matthias, Yoon Chun Hong, White Thomas A, Bajt Saša, Mitraki Anna, Boutet Sebastien, Aquila Andrew, Barty Anton, Forsyth V Trevor, Chapman Henry N, Millane Rick P
Department of Electrical and Computer Engineering, University of Canterbury, Christchurch, New Zealand.
Centre for Free-Electron Laser Science, DESY, Hamburg, Germany.
IUCrJ. 2017 Oct 20;4(Pt 6):795-811. doi: 10.1107/S2052252517014324. eCollection 2017 Nov 1.
Serial diffraction data collected at the Linac Coherent Light Source from crystalline amyloid fibrils delivered in a liquid jet show that the fibrils are well oriented in the jet. At low fibril concentrations, diffraction patterns are recorded from single fibrils; these patterns are weak and contain only a few reflections. Methods are developed for determining the orientation of patterns in reciprocal space and merging them in three dimensions. This allows the individual structure amplitudes to be calculated, thus overcoming the limitations of orientation and cylindrical averaging in conventional fibre diffraction analysis. The advantages of this technique should allow structural studies of fibrous systems in biology that are inaccessible using existing techniques.
在直线加速器相干光源处收集的、来自以液流形式输送的结晶淀粉样纤维的系列衍射数据表明，这些纤维在液流中排列良好。在低纤维浓度下，从单个纤维记录衍射图样；这些图样很微弱，只包含少数几个衍射点。现已开发出确定倒易空间中图样取向并在三维空间中合并它们的方法。这使得能够计算出各个结构振幅，从而克服了传统纤维衍射分析中取向和柱面平均的局限性。这项技术的优势应能使人们对生物学中现有技术无法研究的纤维系统进行结构研究。
