Friedman R M
J Virol. 1968 Oct;2(10):1081-5. doi: 10.1128/JVI.2.10.1081-1085.1968.
Infection of cells treated with guanidine and actinomycin D and then washed to remove the guanidine inhibition of virus growth had no effect on antiviral activity already established by interferon. Protein synthesis in interferon-treated cells infected under these conditions was decreased as compared to control cells similarly treated but not exposed to interferon. In these control cells, analysis by polyacrylamide gel electrophoresis indicated that six proteins were produced during the first hour after guanidine reversal. Five of these proteins have been previously identified as probably being viral in origin. In interferon-treated cells, only a single major protein was produced. Ribonucleic acid (RNA) synthesis by Semliki Forest virus during the first hour after guanidine reversal was markedly depressed by incubation at 42 C, but no inhibition of total virus protein synthesis was seen; this finding suggested that much of the virus protein produced in the first hour after guanidine reversal was carried out by input virus RNA. Interferon was fully active in cells incubated at 42 C. The results suggested that interferon inhibits the production of Semliki Forest virus proteins ordinarily produced under the direction of the virus genome.
用胍和放线菌素D处理细胞，然后洗涤以去除胍对病毒生长的抑制作用，这种感染对干扰素已经建立的抗病毒活性没有影响。与同样处理但未接触干扰素的对照细胞相比，在这些条件下感染的经干扰素处理的细胞中的蛋白质合成减少。在这些对照细胞中，通过聚丙烯酰胺凝胶电泳分析表明，在胍逆转后的第一小时内产生了六种蛋白质。这些蛋白质中的五种先前已被鉴定可能源自病毒。在经干扰素处理的细胞中，只产生了一种主要蛋白质。在胍逆转后的第一小时内，辛德毕斯病毒的核糖核酸（RNA）合成在42℃孵育时明显受到抑制，但未观察到对总病毒蛋白质合成的抑制；这一发现表明，在胍逆转后的第一小时内产生的许多病毒蛋白质是由输入的病毒RNA进行的。干扰素在42℃孵育的细胞中完全有活性。结果表明，干扰素抑制了通常在病毒基因组指导下产生的辛德毕斯病毒蛋白质的产生。
