Bartlett R, Bisset K A
J Med Microbiol. 1979 May;12(2):239-43. doi: 10.1099/00222615-12-2-239.
The rate of reversion from the L-form to the complete bacillus phase of Bacillus licheniformis var. endoparasiticus (BLE)was increased by a factor of c. x20, by growth in the presence of 1% diaminopimelic acid in a well plate, and c. x25 with a 1% hog gastric mucin spread on the plate surface. Saturated riboflavin solution and growth products of staphylococci in wells had a lesser effect. The revertants were subsequently stable when isolated in the absence of additive. The rate of reversion from a spheroplast to a diphtheroid phase was not significantly altered by these additives. These findings are of practical value in studies to distinguish between the BLE sporing bacillus and postulated phases of the organism that include diphtheroid and spheroplast L-forms and debated mycoplasma-like forms.
地衣芽孢杆菌内寄生变种(BLE)从L型回复到完整芽孢杆菌阶段的速率,在微孔板中于1%二氨基庚二酸存在下生长时提高了约20倍,而在平板表面铺有1%猪胃粘蛋白时提高了约25倍。微孔中的饱和核黄素溶液和葡萄球菌生长产物的影响较小。随后在无添加剂情况下分离得到的回复体是稳定的。这些添加剂并未显著改变原生质球回复到类白喉杆菌阶段的速率。这些发现对于区分BLE芽孢杆菌以及该生物体假定阶段(包括类白喉杆菌和原生质球L型以及存在争议的支原体样形态)的研究具有实际价值。