Bartlett R, Bisset K A
J Med Microbiol. 1981 Feb;14(1):97-105. doi: 10.1099/00222615-14-1-97.
Sixty-five samples of blood from patients with rheumatoid arthritis and 94 from control subjects were examined by multiple culture and monthly subculture for periods of up to 33 months to detect the revision stages of Bacillus licheniformis var. endoparasiticus (BLE), which exists as L-forms in blood. Isolation of BLE were obtained more often from the blood of control subjects than from rheumatoid patients during the first 6 months of incubation, when there was clustering of positive cultures within samples. Thereafter, the isolation rate was similar for the two groups and positive cultures were distributed randomly between samples. Isolation of diphtheroid intermediate reversion stages (phase A and B) occurred mainly during the first year of incubation, but isolations of the fully reverted sporing bacillus (phase C) increased in frequency with incubation time, particularly from cultures with a high rate of desiccation during prolonged incubation. The proportion of different phases of BLE amongst the isolates and the distribution of the phases with incubation time were similar for the rheumatoid-arthritis patients and the normal subjects.
对65份类风湿性关节炎患者的血液样本和94份对照受试者的血液样本进行了多次培养，并每月进行传代培养，培养时间长达33个月，以检测地衣芽孢杆菌内寄生变种（BLE）的复壮阶段，该菌在血液中以L型存在。在培养的前6个月内，对照受试者血液中BLE的分离率高于类风湿性关节炎患者，此时样本中的阳性培养物呈聚集状态。此后，两组的分离率相似，阳性培养物在样本间随机分布。类白喉中间复壮阶段（A期和B期）的分离主要发生在培养的第一年，但完全复壮的芽孢杆菌（C期）的分离频率随培养时间增加，特别是在长时间培养中干燥率高的培养物中。类风湿性关节炎患者和正常受试者中BLE不同阶段在分离物中的比例以及各阶段随培养时间的分布相似。
