Reid P, Berg P
J Virol. 1968 Sep;2(9):905-14. doi: 10.1128/JVI.2.9.905-914.1968.
Phage mutants of T4 have been isolated which can multiply only on Escherichia coli strains which contain a missense suppressor which is known to cause the substitution of glycine for arginine in response to the AGA codon. Mutations producing the suppressible phenotype were mapped and shown to occur in six different phage cistrons. Two of the cistrons were concerned with deoxyribonucleic acid synthesis, two were concerned with phage structural components, and two were concerned with functions required for growth in E. coli K-12 but not in E. coli B. The burst size of the different phage mutants grown on strains carrying the same suppressor was dependent upon the efficiency of suppression, which in turn is known to be dependent upon the glycyl-transfer ribonucleic acid synthetase activity.
已分离出T4噬菌体突变体，它们只能在含有错义抑制基因的大肠杆菌菌株上繁殖，已知该抑制基因会导致在AGA密码子的作用下，精氨酸被甘氨酸取代。产生可抑制表型的突变被定位，并显示发生在六个不同的噬菌体顺反子中。其中两个顺反子与脱氧核糖核酸合成有关，两个与噬菌体结构成分有关，另外两个与在大肠杆菌K - 12中生长所需但在大肠杆菌B中不需要的功能有关。在携带相同抑制基因的菌株上生长的不同噬菌体突变体的裂解量取决于抑制效率，而抑制效率又已知取决于甘氨酰转移核糖核酸合成酶的活性。
