Georgopoulos C P
J Bacteriol. 1969 Mar;97(3):1397-402. doi: 10.1128/jb.97.3.1397-1402.1969.
Multiple auxotrophic strains of Bacillus subtilis 168 were tested for joint one-step reversion of two or more auxotrophic markers to the wild-type phenotype. Mu8u5u5, a strain requiring leucine, methionine, and threonine, yielded revertants that grew without added methionine or threonine and proved to have a suppressor gene. When transferred by transformation with deoxyribonucleic acid, this suppressor gene also suppressed the adenine mutation in another strain, Mu8u5u6. The one-step double revertants fell into two distinct classes: strains of class su(+) (I) grow well in broth; strains of class su(+) (II) grow poorly. Strains su(+) (II) tend to revert frequently to the su(+) (I) or su(-) state. Conditional lethal mutants of phage phie were isolated which can grow on the su(+) and not on the su(-) strains.
对枯草芽孢杆菌168的多个营养缺陷型菌株进行了测试，以检测两个或更多营养缺陷型标记向野生型表型的联合一步回复突变。Mu8u5u5是一种需要亮氨酸、蛋氨酸和苏氨酸的菌株，其产生的回复突变体在不添加蛋氨酸或苏氨酸的情况下能够生长，并且被证明具有一个抑制基因。当通过脱氧核糖核酸转化进行转移时，这个抑制基因也抑制了另一个菌株Mu8u5u6中的腺嘌呤突变。一步双回复突变体分为两个不同的类别：su(+) (I)类菌株在肉汤中生长良好；su(+) (II)类菌株生长不良。su(+) (II)类菌株倾向于频繁回复到su(+) (I)或su(-)状态。分离出了噬菌体phie的条件致死突变体，它们可以在su(+)菌株上生长，而不能在su(-)菌株上生长。
