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紫外线照射和热处理对叶绿体的抑制作用。

Inhibition of chloroplasts by UV-irradiation and heat-treatment.

作者信息

Yamashita T, Butler W L

出版信息

Plant Physiol. 1968 Dec;43(12):2037-40. doi: 10.1104/pp.43.12.2037.

Abstract

The site of inhibition in UV-irradiated and heat-treated chloroplasts was examined by using artificial electron donor compounds such as p-phenylenediamine and hydroquinone which donated electrons specifically to photosystem II. In both cases the electron donors restored the photoreduction of nicotinamide adenine dinucleotide phosphate and the restored activity was inhibited by 3-(3,4-dichlorophenyl)-1,1-dimethyl urea. The fluorescence of variable yield was eliminated by both inhibitory treatments and was partially restored by the electron donors in the heat-treated but not the UV-irradiated chloroplasts. The results suggest that the sites of inhibition of UV-radiation and heat treatment are in the photosynthetic electron transport chain between water and photosystem II.

摘要

通过使用诸如对苯二胺和对苯二酚等人工电子供体化合物来检测紫外线照射和热处理的叶绿体中的抑制位点,这些化合物专门向光系统II提供电子。在这两种情况下,电子供体都恢复了烟酰胺腺嘌呤二核苷酸磷酸的光还原,并且恢复的活性受到3-(3,4-二氯苯基)-1,1-二甲基脲的抑制。可变产量荧光在两种抑制处理中都被消除,并且在热处理而非紫外线照射的叶绿体中被电子供体部分恢复。结果表明,紫外线辐射和热处理的抑制位点位于水和光系统II之间的光合电子传递链中。

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