Berman S, Altieri P L, Groffinger A, Lowenthal J P, Formal S B
Appl Microbiol. 1968 Nov;16(11):1779-81. doi: 10.1128/am.16.11.1779-1781.1968.
Of six candidate strains of Shigella prepared in Brain Heart Infusion broth as freeze-dried vaccine, low survival rates were obtained with two of the most promising strains. Survival rates with these two strains were increased to acceptable levels when the organisms were suspended in a medium consisting of 8.2% sucrose, 0.01 M phosphate, 0.07% monosodium glutamate, and 2.5% human serum albumin. Alteration of the freezing temperature did not improve the recovery rates significantly.
在脑心浸液肉汤中制备的六种志贺氏菌候选菌株作为冻干疫苗，其中两种最有前景的菌株存活率较低。当将这些菌株悬浮于由8.2％蔗糖、0.01M磷酸盐、0.07％谷氨酸钠和2.5％人血清白蛋白组成的培养基中时，这两种菌株的存活率提高到了可接受的水平。改变冷冻温度并没有显著提高回收率。
