Yang Lu, Ma Yue, Zhang Yuanxing
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Biologicals. 2007 Oct;35(4):265-9. doi: 10.1016/j.biologicals.2007.03.001. Epub 2007 May 21.
Vibrio anguillarum MVAV6203 is a mutant strain as a candidate of live attenuated vaccine. In vaccine preparation, the freeze-drying conditions of the strain were investigated to improve the survival after freeze-drying, including the protectant, rehydration medium, freezing temperature, and initial cell concentration. Vibrio anguillarum MVAV6203 is sensitive to freeze-drying and the viability was only 0.03% in the absence of protectant. Of the tested protectants, 5% trehalose with 15% skimmed milk gave the highest viability of 34.2%. Higher cell survival was obtained by quick freezing at -80 degrees C than slow freezing at -20 degrees C. Initial cell concentration was another important factor, preferable for 1-3 x 10(10)CFU/ml. The supplementation of 10% skimmed milk in rehydration medium improved obviously freeze-drying viability. The combination of the optimal conditions achieved 51.4% cell viability after freeze-drying.
鳗弧菌MVAV6203是一种作为减毒活疫苗候选株的突变菌株。在疫苗制备过程中，对该菌株的冻干条件进行了研究，以提高冻干后的存活率，包括保护剂、复水培养基、冷冻温度和初始细胞浓度。鳗弧菌MVAV6203对冻干敏感，在没有保护剂的情况下存活率仅为0.03%。在测试的保护剂中，5%海藻糖与15%脱脂牛奶组合的存活率最高，为34.2%。与在-20℃缓慢冷冻相比，在-80℃快速冷冻可获得更高的细胞存活率。初始细胞浓度是另一个重要因素，以1-3×10(10)CFU/ml为宜。在复水培养基中添加10%脱脂牛奶可明显提高冻干存活率。最佳条件组合使冻干后的细胞存活率达到51.4%。
